Acta Chimica Sinica ›› 1997, Vol. 55 ›› Issue (11): 1116-1120. Previous Articles     Next Articles

Original Articles

电喷雾电离质谱测定细胞色素b5及其定点突变蛋白

徐永珍;张一兵;孙雨龙;王韵华;黄仲贤   

  1. 中国科学院上海有机化学研究所;中国科学院生命有机化学国家重点实验室;复旦大学化学系
  • 发布日期:1997-11-15

Molecular weight determination of cytochrome Tb5 and Lb5 and their mutant proteins by electrosprary ionization mass spectrometry technique

XU YONGZHEN;ZHANG YIBING;SUN YULONG;WANG YUNHUA;HUANG ZHONGXIAN   

  • Published:1997-11-15

The determination of molecular weights of cytochrome Tb5 and Lb5 and six mutant proteins has been succedded by using Electrosprary Ionization Mass Spectrometry (ESIMS) technique. The successful site-directed mutagenesis of cytochrom b5 has been proved.

Key words: MOLECULAR WEIGHT, ENZYME HYDROLYSIS, CYTOCHROME, TRYPSIN

CLC Number: