Acta Chimica Sinica ›› 1997, Vol. 55 ›› Issue (6): 585-589. Previous Articles     Next Articles

Original Articles

Hadamard变换显微图象分析III. 单细胞内DNA, RNA和蛋白质的 分析

梅二文;顾文芳;陈观铨;曾云鹗   

  1. 武汉大学分析测试中心.武汉(430072);武汉大学化学系.武汉(430072)
  • 发布日期:1997-06-15

Hadamard transform microscope image analysis III. Analysis of DNA, RNA and protein in a single cell

Mei Erwen;Gu Wenfang;Chen Guanquan;Zeng Yune   

  1. Wuhan Univ., Center of Anal Measur.Wuhan(430072);Wuhan Univ, Dept Chem.Wuhan(430072)
  • Published:1997-06-15

The spectra of single AO and FITC stained cell or nucleus were analyzed by the domestic made instrument of Hadamard transform microscope image analysis. The DNA distribution image in single nucleus of allium cepa epidermal cell was obtained. The instrument was also applied to determine the fluorescence intensity of single cell or nucleus. The results show that the instrument can be used to analyze fluorescence spectra, fluorescence image and distributions and fluorescence intensity of a single cell. The results also show that the instrument is potentially applicable in cytobiology.

Key words: PROTEIN, DEOXYRIBONUCLEIC ACID, IMAGE ANALYSIS, ALLIUM CEPA, FLUOROSPECTROPHOTOMETRY

CLC Number: