Hybrid bilayer membranes were fabricated on monolayer of alkanethiols self-assembled on gold electrode by freezing the monolayer coated with lipid solution for a few minutes. The resulted bilayer membranes were characterized with cyclic voltammetry and ac impedance. The interaction of a pore-forming peptide, melittin with such bilayer membranes was investigated with electrochemical techniques. The presence of hybrid bilayer membranes on electrode shows remarkable insulating properties by greatly attenuating the electron transfer between soluble redox couple. Adsorption of melittin into HBM destroys the insulating capability of HBM, causing membrane resistance to decrease and membrane capacitance to increase, thus accelerating the redox reaction for the negatively charged redox couple Fe(CN)6^3-.

Key words: MERCAPTAN, PHOSPHOLIPID, CYCLOVOLTAMGRAPH, BILAYER THIN FILMS, ELECTROCHEMISTRY, IMPEDANCE, BEE VENOM, INTERACTIONS