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Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (12): 1153-1157. Previous Articles     Next Articles

三维同步偏振荧光光谱研究蛋白质溶液变性时氨基酸残基的发光行为

唐波1, 杜鸣1, 陈蓁蓁1, 张慧1, 沈含熙2   

  1. 1. 山东师范大学化学化工与材料科学学院, 济南, 250014;
    2. 南开大学化学系, 天津, 300071
  • 投稿日期:2003-03-07 修回日期:2003-12-15 发布日期:2014-02-17
  • 通讯作者: 唐波,E-mail:tangb@sdnu.edu.cn;Fax:0531-6180017 E-mail:tangb@sdnu.edu.cn
  • 基金资助:
    国家自然科学基金(No.20335030)和山东省自然科学基金(No.Z2003B01)资助项目.

Studies on Luminescence of Trp and Tyr Residues in Protein Denaturation by Three-dimensional-synchronous-polarized Spectrofluorimetry

TANG Bo1, DU Ming1, CHEN Zhen-Zhen1, ZHANG Hui1, SHEN Han-Xi2   

  1. 1. College of Chemistry, Engineering and Materials Science, Shandong Normal University, Jinan 250014;
    2. Department of Chemistry, Nankai University, Tianjin 300071
  • Received:2003-03-07 Revised:2003-12-15 Published:2014-02-17

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Based on polarizing, synchronous and three-dimensional technologies, a new three-dimensional-synchronous-polarized spectrofluorimetry (TDSPS) was used to distinguish tryptophan (Trp) and tyrosine (Tyr) residues in albumin solution with the advantages of abundant information of three-dimensional technology and high resolution of synchronous spectrofluorimetry. In this paper, the fluorescent spectral changes of Tyr and Trp residues in bovine serum albumin (BSA) and human serum albumin (HSA) were studied by obtaining the characteristic information that had been influenced by various factors (acidic, basic, salt and quenching reagents effects, etc.) with TDSPS. As a result, HSA and BSA could be distinguished.

Key words: three-dimensional-synchronous-polarized spectrofluorimetry(TDSPS), tryptophan(Trp), tyrosine(Tyr), bovine serum albumin(BSA), human serum albumin(HSA)