Construction of a Bio-inspired Signal Transduction System: Activation of Lactate Dehydrogenase Triggered by Lipid Signal Molecules on Bilayer Vesicles

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (13): 1230-1236. Previous Articles Next Articles

仿生信号转导体系的构建:囊泡表面磷脂信号分子诱发的乳酸脱氢酶的激活

田文杰^1,2, 佐佐木善浩¹, 池田笃志¹, 菊池纯一¹, 宋溪明³, 范圣第²

1. 日本奈良先端科学技术大学院大学物质创成科学研究科, 奈良, 630-0192;
2. 大连民族学院生物工程系, 大连, 116600;
3. 辽宁大学化学科学与工程学院, 沈阳, 110036

投稿日期:2003-12-11 修回日期:2004-03-15 发布日期:2014-02-17
通讯作者: 田文杰,菊池纯一,E-mail:jkikuchi@ms.naist.jp;Fax:+81-743-72-6099 E-mail:jkikuchi@ms.naist.jp
基金资助:
日本学术振兴会科学研究基金(No.15205022,2003)资助项目.

Construction of a Bio-inspired Signal Transduction System: Activation of Lactate Dehydrogenase Triggered by Lipid Signal Molecules on Bilayer Vesicles

TIAN Wen-Jie^1,2, SASAKI Yoshihiro¹, IKEDA Atsushi¹, KIKUCHI Jun-ichi¹, SONG Xi-Ming³, FAN Sheng-Di²

1. Graduate School of Materials Science, Nara Institute of Science and Technology, Nara 630-0192, Japan;
2. Department of Biological Engineering, Dalian Nationalities University, Dalian 116600, China;
3. School of Chemical Science and Engineering, Liaoning University, Shenyang 110036, China

Received:2003-12-11 Revised:2004-03-15 Published:2014-02-17

Abstract

An artificial signal transduction system, in which an enzymatic activity was controlled by a lipid signal embedded in a biomembrane model, was constructed.The system was inspired by the biological phosphoinositide (PI)-triggered signal transduction.For this propose, hybrid bilayer vesicles with a mean diameter of 100 nm were formed by combination of dimyristylphosphatidylethanolamine as a lipid signal moiety and a cationic synthetic lipid as a matrix.The aggregate morphology was confirmed by means of transmission electron microscopy and dynamic light scatting measurements, and the vesicles showed a phase transition from gel to liquid-crystalline state at 31.2℃ as evaluated with differential scanning calorimetry.On the cationic vesicular surface, the lipid signal specifically recognized pyridoxal 5'-phosphate as a signal activator to form a signal-signal activator complex.The hydrophobic and the electrostatic interactions were much enhanced in the microenvironment on the vesicular surface to form the binary complex.The resulting binary complex exhibited extremely high binding affinity toward Cu²⁺ ions.Thus, the catalytic activity of lactate dehydrogenase, which was inhibited by binding of Cu²⁺ ion to the enzyme active site on bilayer vesicles, was effectively recovered upon formation of the signal-signal activator complex, reflecting that the binding affinity of the signal-signal activator complex with Cu²⁺ ion overcame that of the enzyme.

Key words: phospholipid signal, vesicle, lactate dehydrogenase, bio-inspired signal transduction

Cite this article

TIAN Wen-Jie, SASAKI Yoshihiro, IKEDA Atsushi, KIKUCHI Jun-ichi, SONG Xi-Ming, FAN Sheng-Di. Construction of a Bio-inspired Signal Transduction System: Activation of Lactate Dehydrogenase Triggered by Lipid Signal Molecules on Bilayer Vesicles[J]. Acta Chimica Sinica, 2004, 62(13): 1230-1236.

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仿生信号转导体系的构建:囊泡表面磷脂信号分子诱发的乳酸脱氢酶的激活

Construction of a Bio-inspired Signal Transduction System: Activation of Lactate Dehydrogenase Triggered by Lipid Signal Molecules on Bilayer Vesicles

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