Direct Chemiluminescent Imaging Method Used to Detect HbA0, HbA1, HbA2 and HbF in Human Erythrocyte Separated by Polyacrylamide Gel Electrophoresis

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (14): 1327-1332. Previous Articles Next Articles

聚丙烯酰胺凝胶电泳直接化学发光成像法分离和检测血液红细胞中的HbA₀,HbA₁,HbA₂和HbF

申素辉¹, 翟所迪², 欧阳津¹, 张现化¹, 张会英³

1. 北京师范大学化学系, 北京, 100875;
2. 北京大学第三医院, 北京, 100083;
3. 北京积水潭医院, 北京, 100035

投稿日期:2003-10-10 修回日期:2004-03-04 发布日期:2014-02-17
通讯作者: 欧阳津,E-mail:jinoyang@bnu.edu.cn;Fax:010-62799838 E-mail:jinoyang@bnu.edu.cn
基金资助:
国家自然科学基金(No.20375005)资助项目.

Direct Chemiluminescent Imaging Method Used to Detect HbA₀, HbA₁, HbA₂ and HbF in Human Erythrocyte Separated by Polyacrylamide Gel Electrophoresis

SHEN Su-Hui¹, ZHAI Suo-Di², OUYANG Jin¹, ZHANG Xian-Hua¹, ZHANG Hui-Ying³

1. Department of Chemistry, Beijing Normal University, Beijing 100875;
2. The Third Hospital of Peking University, Beijing 100083;
3. Beijing Jishuitan Hospital, Beijing 100875

Received:2003-10-10 Revised:2004-03-04 Published:2014-02-17

Abstract

A new method of direct chemiluminescent imaging to detect proteins separated by polyacrylamide gel eletrophoresis was established.This method has been applied to the separation and detection of hemoglobins in erythrocyte of healthy voluntary people and umbilical cord blood.Serum proteins were also detected using hemoglobin as probe.Compared with the traditional Coomassie brilliant blue R250 stain method, direct chemiluminescent imaging offers a fast, convenient and inexpensive method with high sensitivity and selectivity.Furthermore, the effect of human serum albumin on the electrophoretic result of the hemoglobin was studied.The chemiluminescent imaging conditions have been optimized in the experiment.

Key words: direct chemiluminescent imaging, polyacrylamide gel electrophoresis, hemoglobin

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SHEN Su-Hui, ZHAI Suo-Di, OUYANG Jin, ZHANG Xian-Hua, ZHANG Hui-Ying. Direct Chemiluminescent Imaging Method Used to Detect HbA₀, HbA₁, HbA₂ and HbF in Human Erythrocyte Separated by Polyacrylamide Gel Electrophoresis[J]. Acta Chimica Sinica, 2004, 62(14): 1327-1332.

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References

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聚丙烯酰胺凝胶电泳直接化学发光成像法分离和检测血液红细胞中的HbA₀,HbA₁,HbA₂和HbF

Direct Chemiluminescent Imaging Method Used to Detect HbA₀, HbA₁, HbA₂ and HbF in Human Erythrocyte Separated by Polyacrylamide Gel Electrophoresis

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聚丙烯酰胺凝胶电泳直接化学发光成像法分离和检测血液红细胞中的HbA0,HbA1,HbA2和HbF