Detection of Hepatitis B Surface Antigen Using Immunovoltammetry with Nano-Magnetic Microsphere

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (24): 2447-2450. Previous Articles

纳米磁性微球免疫伏安法测定乙肝表面抗原

程琼^1,2, 彭图治¹, 刘爱丽¹

1. 浙江大学化学系, 西溪校区, 杭州, 310028;
2. 嘉兴学院生物与化工学院, 嘉兴, 314001

投稿日期:2004-05-25 修回日期:2004-08-24 发布日期:2014-02-17
通讯作者: 彭图治,E-mail:tzp@zju.edu.cn;Tel:0571-88273689;Fax:0571-88273690. E-mail:tzp@zju.edu.cn
基金资助:
国家自然科学基金(Nos.29975024,20275034)与浙江省重点科技计划(No.2003C21024)资助项目.

Detection of Hepatitis B Surface Antigen Using Immunovoltammetry with Nano-Magnetic Microsphere

CHENG Qiong^1,2, PENG Tu-Zhi¹, LIU Ai-Li¹

1. Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028;
2. College of Biological and Chemical Engineering, Jiaxing College, Jiaxing 314001

Received:2004-05-25 Revised:2004-08-24 Published:2014-02-17

Abstract

A novel method for detecting hepatitis B surface antigen (HBsAg) using nano-magnetic polymer carboxyl microsphere was reported. The surface of the nano-magnetic polymer carboxyl microsphere covalently bound with hepatitis B surface antibody (HBsAb), and then captured HBsAg and hepatitis B secondary antibody labeled with horseradish peroxide enzyme. The microsphere with sandwich complex was separated magnetically from sample solution and the enzyme labeled at the complex catalyzed the reaction of 2-aminohydroxybenzene and hydrogen peroxide in the buffer solution. The electroactive product 2-hydroxy-3-aminophenoxazine was detected using differential pulse voltammetry. The peak current was linear with the concentration of HBsAg in the range of 0.2～1.0 and 1.0～500 ng·mL^-1 respectively. The detection limit was found to be 0.06 ng·mL^-1. The method has been used to detect the concentration of hepatitis B virus in human serum and the sensitivity was much higher than that of enzyme-linked immunosorbent assay (ELISA) used in diagnostic analysis.

Key words: nano-magnetic functional microsphere, hepatitis B surface antigen, immunoassay, electrochemical detection

CLC Number:

O657.1

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CHENG Qiong, PENG Tu-Zhi, LIU Ai-Li. Detection of Hepatitis B Surface Antigen Using Immunovoltammetry with Nano-Magnetic Microsphere[J]. Acta Chimica Sinica, 2004, 62(24): 2447-2450.

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纳米磁性微球免疫伏安法测定乙肝表面抗原

Detection of Hepatitis B Surface Antigen Using Immunovoltammetry with Nano-Magnetic Microsphere

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