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Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (1): 71-74. Previous Articles     Next Articles

Original Articles

二(2-苯并咪唑亚甲基)胺合铜(II)配合物与DNA作用方式的光谱研究

周庆华，杨频*   

  1. (山西大学分子科学研究所 化学生物学与分子工程教育部重点实验室 太原 030006)
  • 投稿日期:2004-04-28 修回日期:2004-09-03 发布日期:2010-12-10
  • 通讯作者: 杨频

Study on the Binding Mode of a Cu(II) Complex with DNA

ZHOU Qing-Hua, YANG Pin*   

  1. (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science,
    Shanxi University, Taiyuan 030006)
  • Received:2004-04-28 Revised:2004-09-03 Published:2010-12-10
  • Contact: YANG Pin

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The interaction of the LCu(ClO4)2 [L＝bis(2-benzimidazolylmethyl)amine] with calf thymus DNA has been investigated using UV spectra, fluorescent spectra, and viscosity. Hypochromism was observed in the UV spectra of DNA in the presence of the complex, and decreased fluorescence was observed for complex with native and denatured DNA addition. The relative viscosity of DNA decreased with addition of the complex. The Scatchard plots show uncompetitive inhibition of EB binding to DNA. The experimental results show that the mode of binding of the complex to DNA is electrostatical binding.

Key words: bis(2-benzimidazolylmethyl)amine, calf thymus DNA, Scatchard plot, electrostatical binding