Chronopotentiometry Based on Nano-Au Labeled Aggregate Enlargement Used for the Immunoassay of Complement C3

Acta Chimica Sinica ›› 2005, Vol. 63 ›› Issue (22): 2093-2097. Previous Articles Next Articles

Original Articles

纳米金聚集复合物放大的计时电位法测定补体C₃

周谷珍¹,2，李继山¹，蒋健晖¹，沈国励*^,1，俞汝勤¹

(¹湖南大学化学化工学院化学生物传感与计量学国家重点实验室长沙 410082)
(²湖南文理学院化学化工系常德 415000)

投稿日期:2005-03-09 修回日期:2005-07-29 发布日期:2010-12-10
通讯作者: 沈国励

Chronopotentiometry Based on Nano-Au Labeled Aggregate Enlargement Used for the Immunoassay of Complement C₃

ZHOU Gu-Zhen¹,2, LI Ji-Shan¹, JIANG Jian-Hui¹, SHEN Guo-Li*^,1, YU Ru-Qin¹

(¹ State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,
Hunan University, Changsha 410082)
(² Department of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000)

Received:2005-03-09 Revised:2005-07-29 Published:2010-12-10
Contact: SHEN Guo-Li

Abstract

A novel electrochemical immunoassay method based on Au nanoparticle labeled immunocomplex enlargement used for detection of complement C₃ was proposed in this paper. When the aggregates formed from nano-Au labeled goat-anti-human C₃ and nano-Au labeled rabbit-anti-goat IgG were immobilized on the electrode surface by sandwich method (antibody/antigen/aggregate), the electrochemical signal of the electrode was enlarged greatly. Fabrication of the immunoelectrode and the effects of experimental conditions on the performances of immunoassay, such as concentration of protein A, pH of incubating solution and the ratio of nano-Au labeled goat-anti-human C₃ to nano-Au labeled rabbit-anti-goat IgG, were discussed respectively. The formed immunosensor could quantitatively determine complement C₃ in the range of 0.12～117.3 ng/mL, and the detection limit was 0.02 ng/mL.

Key words: chronopotentiometry, complement C₃, nano-Au, immunoassay

Cite this article

ZHOU Gu-Zhen^1,2, LI Ji-Shan, JIANG Jian-Hui, SHEN Guo-Li*^,1, YU Ru-Qin¹. Chronopotentiometry Based on Nano-Au Labeled Aggregate Enlargement Used for the Immunoassay of Complement C₃[J]. Acta Chimica Sinica, 2005, 63(22): 2093-2097.

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纳米金聚集复合物放大的计时电位法测定补体C₃

Chronopotentiometry Based on Nano-Au Labeled Aggregate Enlargement Used for the Immunoassay of Complement C₃

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纳米金聚集复合物放大的计时电位法测定补体C3