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Acta Chimica Sinica ›› 2006, Vol. 64 ›› Issue (18): 1897-1903. Previous Articles     Next Articles

Original Articles

全二维气相色谱/飞行时间质谱(GC×GC/TOFMS)用于烟叶中挥发、半挥发性碱性化合物的组成研究

李海锋1,2,4, 钟科军*,1, 路鑫2, 白长敏2, 黄建国1, 鹿洪亮3, 马晨菲2, 朱书奎2, 孔宏伟2, 赵明月3, 谢剑平

  1. (1湖南常德卷烟厂技术中心 常德 415000)
    (2中国科学院大连化学物理研究所 国家色谱研究分析中心 大连 116023)
    (3郑州烟草研究院 郑州 450000)
    (4沈阳农业大学 沈阳 110161)
  • 投稿日期:2005-11-08 修回日期:2006-03-09 发布日期:2006-09-28
  • 通讯作者: 许国旺

Analysis of Volatile and Semi-volatile Alkaline Compounds in Tobacco Leaf by Using Comprehensive Two-Dimensional Gas Chromatogra-phy/Time-of-Flight Mass Spectrometry

LI Hai-Feng1,2,4; ZHONG Ke-Jun*,1; LU Xin2; BAI Chang-Min2; HUANG Jian-Guo1; LU Hong-Liang3; MA Chen-Fei2; ZHU Shu-Kui2; KONG Hong-Wei   

  1. (1 Center of Science and Technology, Changde Cigarette Factory, Changde 415000)
    (2 National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023)
    (3 Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000)
    (4 Shenyang Agriculture University, Shenyang 110161)
  • Received:2005-11-08 Revised:2006-03-09 Published:2006-09-28
  • Contact: XU Guo-Wang

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Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC× GC-TOFMS) has been applied for characterization of volatile and semi-volatile alkaline compounds in oriental tobacco leaf. Simultaneous distillation extraction (SDE) was used as the sample extraction method. Two types of column set were compared, and appropriate GC×GC separation conditions were suggested. The results showed that the peaks detected by GC×GC method were far more than that by 1DGC. The separation behavior on 2D plot of different kinds of compounds was investigated. TOFMS library search combining structured chromatogram of GC×GC was used to identify the nitrogen-containing compounds in tobacco leaf. A total of 92 alkaline compounds were tentatively identified. The results showed that GC×GC/TOFMS method is suitable for separation and identification of complex system.

Key words: tobacco leaf, organic alkaline compound, comprehensive two dimensional gas chromatography (GC×GC), time of flight mass spectrometry (TOFMS)