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Acta Chimica Sinica ›› 2007, Vol. 65 ›› Issue (14): 1353-1356. Previous Articles     Next Articles

Original Articles

基于二氧化硅微颗粒促细胞增殖效应的基因转染新方法

林霞1,2,3,4, 何晓晓1,3,4,5, 王柯敏*,1,2,3,4, 谭蔚泓1,2,3,4   

  1. (1湖南大学1化学生物传感与计量学国家重点实验室
    2化学化工学院 3生物纳米与分子工程湖南省重点实验室 4湖南大学生物医学工程中心 5生命科学与技术研究院 长沙 410082)
  • 投稿日期:2006-09-30 修回日期:2007-01-30 发布日期:2007-07-28
  • 通讯作者: 王柯敏

A New Gene Transfection Based on the Promotion Effect of Silica Microparticles on Cell Proliferation

LIN Xia1,2,3,4; HE Xiao-Xiao1,3,4,5; WANG Ke-Min*,1,2,3,4; TAN Wei-Hong1,2,3,4   

  1. (1 State Key Laboratory of Chemo/Biosensing and Chemometrics, )
    (2 College of Chemistry and Chemical Engineering, 3 Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, 4 Biomedicine Engineering Center of Hunan University, 5 Institute of Life Science and Biotechnology, Hunan University, Changsha 410082)
  • Received:2006-09-30 Revised:2007-01-30 Published:2007-07-28
  • Contact: WANG Ke-Min

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Promotion effect of silica microparticles (SiMP) on cell proliferation was reported. Taking advantage of this effect, a new gene transfection method utilizing SiMP as transfection helpmates was developed. An MTT assay was performed to demonstrate the promotion effect of SiMP on cell proliferation. Based on this effect, SiMP acting as transfection helpmates successfully enhanced poly-L-lysine (PLL) mediated gene transfection. This transfection method showed an above 5-fold increase in transfection efficiency compared with the PLL method that lacked the assistance of SiMP in the delivery of plasmid pEGFP, which encodes green fluorescence protein into COS-7 cells. This new transfection method provides a new strategy to increase transfection efficiency from the angle of promoting the target cell proliferation and viability.

Key words: silica microparticle, promotion of cell proliferation, gene transfection, transfection helpmate