Study on β-Sheet Structure Formed by Self-assembly of Fibroin Crystalline Typical Peptides Using Thioflavine T Fluorescence Probe

Abstract

To study the self-organization/self-assembly mechanism of silkworm fibroin based material, four kinds of structurally simple fibroin crystalline peptides, GXGAGAGXGA (X: A, S, Y, V), were designed and then incubated for 1～15 d to assemble by themselves. Their abilities to form β-sheet structure were studied by ThT fluorescence spectral analysis and urea denaturation methods. Results showed that: GA could form β-sheet after incubation for 1 d; after the peptide was incubated for 12, 8, 14 and 13 d respectively (in the order of A, S, Y and V), the ratio of β-sheet structure in the aggregation tended to be stable. Among the four peptides GS had the highest β-sheet content, GA took the second place, GY and GV had the lowest content. The presence of urea in peptide solution had an obvious effect on the formation of β- sheet. Urea (1 mol/L) could stop the forming of β-sheet structure from GS, GY and GV in the process of their self-assembly. When the concentration of urea was over 2 mol/L, the formation of β-sheet structure from GA could be affected, and the content of β-sheet decreased proportionally with increase of urea concentration.

Key words: fibroin crystalline peptide, β-sheet, ThT fluorescence probe, urea

Cite this article

WANG Jian-Nan*^,1,2; LU Chang-De³; BAI Lun*^,1. Study on β-Sheet Structure Formed by Self-assembly of Fibroin Crystalline Typical Peptides Using Thioflavine T Fluorescence Probe[J]. Acta Chimica Sinica, 2007, 65(2): 111-115.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	Chang-An Liu, Shi-Bo Hong, Bei Li. Molecular Dynamics Simulation of the Stability Behavior of Graphene in Glycerol/Urea Solvents in Liquid-Phase Exfoliation [J]. Acta Chimica Sinica, 2021, 79(4): 530-538.
[2]	Du Chongyang, Chen Yaofeng. ZnEt₂ Promoted Hydrosilylation of CO₂ and Formylation or Urealation of Amines with CO₂ as a C1 Building Block [J]. Acta Chimica Sinica, 2020, 78(9): 938-944.
[3]	Song Qiusheng, Zhou Wen, Wu Xinmin, Wu Fan. Dual Stimulus-responsive Fluorescence Behavior and Mechanism of P(NIPAM-co-RhBHA)-NP [J]. Acta Chim. Sinica, 2016, 74(5): 435-440.
[4]	Liu Mengying, Che Jianing, Wu Weihong, Lu Yunxiang, Peng Changjun, Liu Honglai, Lu Hao, Yang Qiang, Wang Hualin. Extraction of Copper from Aqueous Solution with Functional Ionic Liquids: Experiment and Theoretical Calculation [J]. Acta Chim. Sinica, 2015, 73(2): 116-125.
[5]	Zhang Qi, Fang Hongxia, Zhang Huili, Qin Dan, Hong Zhi, Du Yong. Co-crystal between Nitrofurantion and Urea Investigated by Terahertz Spectroscopy and Density Functional Theory [J]. Acta Chim. Sinica, 2015, 73(10): 1069-1073.
[6]	Lin Qi, Zhu Xin, Chen Pei, Fu Yongpeng, Zhang Youming, Wei Taibao. Copper Complex Based Chemosensor which Could Colorimetrically Detect CN^- in Water with Specific Selectivity and High Sensitivity [J]. Acta Chimica Sinica, 2013, 71(11): 1516-1520.
[7]	Chen Zhanguo, Wang Dan, Li Yanan, Wang Yingjie, Hu Junli, Xia Wei. Synthesis of Functionalized Enamines (I): theα,β-Vicinal Bromoamine Compounds Converted into α,β-Dehydroamino Derivatives Catalyzed by Thiourea in Mixture Solvent [J]. Acta Chimica Sinica, 2012, 70(21): 2236-2245.
[8]	Gu Xuelin, Yang Jiping. Study on the Prepolymer Components of High-Performance Self-Healing Microcapsules [J]. Acta Chimica Sinica, 2012, 70(06): 753-758.
[9]	Hao Zhixian, Cheng Yiyi, Wang Lele, Wang Xiaogang, Zhu Zhirong, Gan Lihua, Xu Zijie, Chen Longwu. Synthesis of Urea-Formaldehyde Resin Microspheres under Surfactant Conditions [J]. Acta Chimica Sinica, 2012, 70(03): 331-338.
[10]	HAO Zhi-Xian, LUO Hao, CHENG Yi-Yi, WANG Xiao-Gang, WANG Li-Jun, ZHU Zhi-Rong, GAN Li-Hua, XU Zi-Jie, CHEN Long-Wu. Synthesis of Silica Microspheres under Hybridization of Urea-Formaldehyde Resin in the Presence of Surfactants [J]. Acta Chimica Sinica, 2011, 69(21): 2531-2538.
[11]	LV Jing, JIANG Yong-Jun, YU Qing-Sen, ZOU Jian-Wei. Molecular Docking and Molecular Dynamics Simulations of Inhibitors Binding to Jack Bean Urease [J]. Acta Chimica Sinica, 2011, 69(20): 2427-2433.
[12]	ZHAO Hua-Rong, WANG Hai-Yan, LU Feng-Ping. An Efficient Two-Step Synthesis of N-Substituted Thioureas in Functional Ionic Liquild [J]. Acta Chimica Sinica, 2011, 69(19): 2293-2298.
[13]	ZHAO Bin, ZHAO Su-Qing, ZHANG Kun, LIU Duan, SU Juan. Preparation and Characterization of Thiourea Modified Water-Soluble Cd Doped ZnO Quantum Dots [J]. Acta Chimica Sinica, 2011, 69(07): 777-782.
[14]	. A Novel Complex of Thiourea Receptor with Gadolinium Ion as a Compound Selective Recognition of Fluoride Ion [J]. Acta Chimica Sinica, 2011, 69(06): 709-712.
[15]	ZHANG Lin-Qun, GU Wei-Jin, CHEN Chang-Xin, WANG Bing-Xiang. Synthesis and Fluorescence Properties of the N-Cycl[3,2,2]azineacyl Urea Derivative [J]. Acta Chimica Sinica, 2010, 68(19): 1981-1985.

Thioflavine T荧光探针法研究丝素结晶区典型肽段自聚集的β-片层结构