Iron-binding proteome of rat liver were enriched by Fe3＋-immobilized silica nanomaterials and analyzed by HPLC-MS. More proteins were identified with enrichment of the Fe3＋-immobilized silica nanomaterials than those with normal silica nanomaterials when the starting proteins with the same amount and the same conditions of absorption and elution were used. Finally, 42 iron-binding proteins were identified, which were mainly referred to enzymes of metabolism, members of respiratory chain, proteins involved in oxidation-reduction, transporters and hemoglobins.

Key words: magnetic nanomaterial, iron-binding, proteome