In order to understand the mechanism of the transition between different mitochondrial functional states, an in-situ study of the stability of myelin figure composed of cardiolipin was performed by changing the electrolyte concentration of systems. The methods of changing electrolyte concentration are the solvent-evaporation method and the micro-injection method. It was found that, even without protein regulation, the morphology of cardiolipin membrane could be reversibly mediated by only increasing or decreasing the electrolyte concentration. Moreover, the myelin crimple was related to the rising of zeta potential on the surface of membrane.

Key words: cardiolipin, myelin figure, stability of structure, morphology of self-assembly