Studies on Preparation of Oligogalacturonic Acids from Apple Pectin and Analysis by ESI-MS

Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (15): 1525-1531. Previous Articles Next Articles

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苹果果胶活性寡聚半乳糖醛酸的分离制备及其ESI-MS分析研究

徐溪，李潇，黄琳娟，王仲孚

(西北大学生命科学学院西北大学糖生物学与糖工程研究中心西安 710069)

投稿日期:2010-03-04 修回日期:2010-04-28 发布日期:2010-05-16
通讯作者: 王仲孚 E-mail:wangzhfnwu@163.com
基金资助:
国家高技术研究发展计划;国家高技术研究发展计划;教育部新世纪优秀人才支持计划;陕西省重点实验室重点科研计划项目

Studies on Preparation of Oligogalacturonic Acids from Apple Pectin and Analysis by ESI-MS

XU Xi, LI Xiao, HUANG Lin-Juan, WANG Zhong-Fu

(Glycobiology and Glycotechnology Research Center, Life Science College, Northwest University, Xi an 710069)

Received:2010-03-04 Revised:2010-04-28 Published:2010-05-16

Abstract

A low degree of esterification pectic acid was obtained by sodium hydroxide treatment of apple pectin. Pectic acid was depolymerized with a commercially available pectinase and a mixture of oligo-GalA (DP2-8) was pooled together, the mixture was separated by a low-pressure anion-exchange resin column chromatography to obtain pure oligo-GalA. The degradation conditions were optimized as enzymatic reaction at 40 ℃ for 1 h, oligo-GalA monomers in the range of 2～5 with a purity degree over 92% analyzed by TLC and ESI-MS were prepared (yield of 37%). The results indicated that there also existed dimer molecular ion peaks along with oligogalacturonic acid monomer molecular ion peaks, and the presence of the dimer were identified by ESI-MS/MS.

Key words: apple pectin, oligogalacturonic acid, anion-exchange resin, ESI-MS

Cite this article

XU Xi, LI Xiao, HUANG Lin-Juan, WANG Zhong-Fu. Studies on Preparation of Oligogalacturonic Acids from Apple Pectin and Analysis by ESI-MS[J]. Acta Chimica Sinica, 2010, 68(15): 1525-1531.

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苹果果胶活性寡聚半乳糖醛酸的分离制备及其ESI-MS分析研究

Studies on Preparation of Oligogalacturonic Acids from Apple Pectin and Analysis by ESI-MS

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