Effect of Biological Activity of Nano Eu2O3 on HEK-293T Cells

Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (01): 106-110. Previous Articles

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纳米Eu₂O₃对HEK-293T细胞的生物活性影响

周雪萍¹，侯惠智²，李英奇¹^{,2，杨斌盛*^{,1，杨频*^,1}}

(¹山西大学分子科学研究所化学生物学与分子工程教育部重点实验室太原 030006)
(²山西大学化学化工学院太原 030006)

投稿日期:2010-06-25 修回日期:2010-09-01 发布日期:2009-09-19
通讯作者: 李英奇 E-mail:wkyqli@sxu.edu.cn
基金资助:
国家自然科学基金项目;山西省自然科学基金;山西省自然科学青年基金;太原市大学生创新创业计划

Effect of Biological Activity of Nano Eu₂O₃ on HEK-293T Cells

Zhou Xueping¹ Hou Huizhi² Li Yingqi¹^{,2 Yang Binsheng*^{,1 Yang Pin*^,1}}

(¹Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006)
(²Department of Chemistry, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006)

Received:2010-06-25 Revised:2010-09-01 Published:2009-09-19
Contact: LI-Ying-Qi E-mail:wkyqli@sxu.edu.cn

Abstract

The research on the biological effect of rare earth compound has aroused wide concerns. In this paper, the results reveal that nano Eu₂O₃ can be internalized by live HEK-293T cells and accumulate in the perinuclear region in (Dulbecco s Modified Eagle Medium) DMEM with 10% (Fetal bovine serum) FBS by laser scanning confocal microscope. In vitro culture conditions, the effects of nano Eu₂O₃on cell viability and the cell cycle were investigated using flow cytometry. The results showed that cell activity was free from nanoparticles with concentration less than 200 μg•mL^－1. When nanoparticles concentration is more than 400 μg•mL^－1, the cell activity was inhibited. Eu₂O₃ nanoparticles at low concentration (≤50 μg•mL^－1) had no effect on the cell cycle, while high concentrations (≥200 μg•mL^－1) cell cycle was significantly inhibited, which showed DNA synthesis in the late stagnation. These results indicated that nano Eu₂O₃ on HEK-293T cells have obvious biological effects.

Key words: nano Eu₂O₃, biological activity, HEK-293T cells

Cite this article

ZHOU Xue-Ping, HOU Hui-Zhi, LI Ying-Qi, YANG Bin-Sheng, YANG Pin. Effect of Biological Activity of Nano Eu₂O₃ on HEK-293T Cells[J]. Acta Chimica Sinica, 2011, 69(01): 106-110.

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纳米Eu₂O₃对HEK-293T细胞的生物活性影响

Effect of Biological Activity of Nano Eu₂O₃ on HEK-293T Cells

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纳米Eu2O3对HEK-293T细胞的生物活性影响