Under microwave assisted heating, uridine imprinted polymer was prepared rapidly using uridine as template, acrylamide (AA) as monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The results indicated that the polymerization time was shorten significantly, only one eight of the conventional heating. Infrared spectroscopy and scanning electron microscopy were used to characterize the structure and morphology of the imprinted polymer, and the results showed that the imprinted polymer was coated on the surface of silica microspheres successfully. Well-dispersed particles are observed and the thickness of the imprinted shell is about 100 nm. Combined with high liquid chromatography technique, on-line imprinted enrichment and determination of nucleotide ingredients from the extracting solution of isatidis root were performed successfully.

Key words: microwave assisted, uridine, imprinted polymer, on-line enrichment