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Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (15): 1795-1802. Previous Articles     Next Articles

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附子配伍过程中二萜类生物碱在Caco-2小肠吸收细胞模型中吸收转运的UPLC/MS研究

韩天娇1,3, 宋凤瑞1, 刘忠英2, 皮子凤*,1, 刘志强*,1, 刘淑莹1   

  1. (1中国科学院长春应用化学研究所 长春 130022)
    (2吉林大学药学院 长春 130021)
    (3中国科学院研究生院 北京 100039)
  • 投稿日期:2011-01-26 修回日期:2011-03-01 发布日期:2011-03-28
  • 通讯作者: 刘志强 E-mail:liuzq@ciac.jl.cn

Studies of the Intestinal Transport of the Diterpenoid Alkaloids in the Aconitum carmichaeli Combined with Different Medicinal Herbs in a Caco-2 Cell Culture System with UPLC/MS

Han Tianjiao1,3; Song Fengrui1; Liu Zhongying2; Pi Zifeng*,1; Liu Zhiqiang*,1; Liu Shuying1   

  1. (1 Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
    (2 School of Pharmacy, Jilin University, Changchun 130021)
    (3 Graduate School of the Chinese Academy of Sciences, Beijing 100039)
  • Received:2011-01-26 Revised:2011-03-01 Published:2011-03-28

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A UPLC/MS method has been established to analyze the diterpenoid alkaloids transportation in the Aconitum carmichaeli with the Caco-2 cell monolayer. Different combination herbs water extracts including Radix Glycyrrhizae, Rhizoma Zingiberis, Rhizoma Pinelliae or Bulbus Frltillariae Thunbergii have been mixed with the same concentration of the Aconitum carmichaeli alkaloids extract solution. The mixing ratio was converted into their medicinal herbs mass of 1 to 1. The results demonstrated that the bioavailability of the diterpenoid alkaloids especially the diester-diterpenoid alkaloids has been decreased by the adding of the Radix Glycyrrhizae, Rhizoma Zingiberis or Rhizoma Pinelliae extracts, which are the most toxicity compounds in the Aconitum carmichaeli. However, the bioavailability of the diterpenoid alkaloids in the absorption and efflux has been increased by the adding of the Bulbus Frltillariae Thunbergii extract, respectively.

Key words: Caco-2 cell monolayer, UPLC/MS, diterpenoid alkaloids, Aconitum carmichaeli, Radix Glycyrrhizae, Rhizoma Zingiberis, Rhizoma Pinelliae, Bulbus Frltillariae Thunbergii