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Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (20): 2486-2492. Previous Articles     Next Articles

Full Papers

生物亲和性壳聚糖-二氧化硅复合纳米粒子的合成及其吸附DNA特性研究

张李娜1，郭志慧1，郑行望*,1，汪绒1，孟美荣1，屈颖娟1，刘全宏2，姚莎1   

  1. (1陕西省生命分析化学重点实验室 陕西师范大学化学与材料科学学院 西安 710062)
    (2陕西师范大学生命科学学院 西安 710062)
  • 投稿日期:2011-03-21 修回日期:2011-06-22 发布日期:2011-06-30
  • 通讯作者: 郑行望 E-mail:zhengxw@snnu.edu.cn
  • 基金资助:

    国家自然科学基金项目;国家文物局项目

Synthesis of Biocompatible Chitosan-doped SiO2 Nanoparticles and Its Application for DNA Adsorption

Zhang Lina1 Guo Zhihui1 Zheng Xingwang*,1 Wang Rong1 Meng Meirong1 Qu Yingjuan1 Liu Quanhong2 Yao Sha1   

  1. (1 Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Xi′an 710062)
    (2 College of Life Sciences, Shaanxi Normal University, Xi′an 710062)
  • Received:2011-03-21 Revised:2011-06-22 Published:2011-06-30
  • Contact: ZHENG Xing-Wang E-mail:zhengxw@snnu.edu.cn

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A novel mesoporous chitosan-silica nanoparticles was synthesized with the water-in-oil (W/O) reverse microemulsion method. By characterizing this nanoparticles with transmission electron microscopy, IR spectra and the zeta potential experiments, it was found that those nanoparticles were spherical and uniform with 50 nm size and presented the mesoporous structure. Based on these findings, the adsorption ability of this nanoparticles for calf thymus DNA was studied. The relating results showed that this nanoparticles had a stronger adsorption ability to DNA in PBS buffer (pH＝6), they also presented the better biocompatibility for cell and could be used for protect DNA from Dnase I cleavage.

Key words: chitosan-silica nanoparticles, reverse microemulsion, calf thymus DNA