Separation and Purification of Hemoglobin by Using Liquid-Solid Extraction System of Cu(II)IDAPEG6000-Tween 80-(NH_4)_2SO_4

Acta Chimica Sinica ›› 2002, Vol. 60 ›› Issue (5): 859-865. Previous Articles Next Articles

Original Articles

PEG6000修饰物混合吐温80－硫酸铵盐的液－固萃取体系分离纯化血红蛋白

沈静茹;孙小梅;雷灼霖;李步海;秦晓蓉

中南民族学院化学系,武汉(430074);武汉科技大学化学与环境工程学院,武汉 (430071)

发布日期:2002-05-15

Separation and Purification of Hemoglobin by Using Liquid-Solid Extraction System of Cu(II)IDAPEG6000-Tween 80-(NH_4)_2SO_4

Shen Jingru;Sun Xiaomei;Lei Zhuolin;Li Buhai;Qin Xiaorong

Department of Chemistry, South-Central University for Ethnic Communities,Wuhan(430074);School of Chemical and Environmental Engineering, Wuhan University of Science and Technology,Wuhan(430071)

Published:2002-05-15

Abstract

The method of separation and purification of hemoglobin by using liguid-solid extraction system of Cu(II)-IDAPEG 6000-tween 80-(NH_4) _2SO_4 was studied. The influences of conditions such as the amount of Cu(II)IDAPEG 6000, concentrations of Tween 80 and salt, acidity, temperature, etc., were studied. It was simple and rapid to extract pig hemoglobin, human hemoglobin and bovine hemoglobin from blood using this extraction systems, with high extraction capacity and extraction rate. The one-solid phase extraction rate was more than 99%, and the one-step reextraction rate was 75%. Hemoglobin purification fold was about 20.00. The purity of pig hemoplobin (PHb) in the sample reached 99%.

Key words: POLYGLYCOL, EXTRACTION, HEMOGLOBIN, ISOLATION, PURIFICATION, TWEEN 80, AMMONIUM SULFATE

CLC Number:

O658

Cite this article

Shen Jingru;Sun Xiaomei;Lei Zhuolin;Li Buhai;Qin Xiaorong. Separation and Purification of Hemoglobin by Using Liquid-Solid Extraction System of Cu(II)IDAPEG6000-Tween 80-(NH_4)_2SO_4[J]. Acta Chimica Sinica, 2002, 60(5): 859-865.

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PEG6000修饰物混合吐温80－硫酸铵盐的液－固萃取体系分离纯化血红蛋白

Separation and Purification of Hemoglobin by Using Liquid-Solid Extraction System of Cu(II)IDAPEG6000-Tween 80-(NH_4)_2SO_4

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