Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (3): 185-190.     Next Articles

Original Articles

氢化松香酸钠与明胶蛋白质相互作用的荧光光谱法研究

唐世华* 李景权 雷福厚

  

  1. (广西民族大学化学与生态工程学院 南宁 530006)

  • 投稿日期:2008-07-15 修回日期:2008-08-19 发布日期:2009-02-14
  • 通讯作者: 唐世华

Fluorescence Spectral Study on Interaction of Hydrogenated Sodium Rosinate with Gelatin

Tang, Shihua* Li, Jingquan Lei, Fuhou   

  1. (College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006)
  • Received:2008-07-15 Revised:2008-08-19 Published:2009-02-14
  • Contact: Tang, Shihua

The interaction between hydrogenated sodium rosinate (HSR) and gelatin in aqueous solution was investigated by fluorescence spectroscopy, synchronous fluorescence spectroscopy, resonance light scattering (RLS) and UV-Vis spectra at pH 10..80 and different temperatures.. The experimental result showed that HSR and gelatin formed a 1∶1 aggregate.. The equation for calculating the binding constant in of HSR for to gelatin was introduced based on the relationships between the fluorescence enhancement of gelatin and the concentration of HSR.. According to the fluorescence enhancement equation, the binding constants of the aggregate at different temperatures and the thermodynamic parameters at corresponding temperatures were obtained.. It was suggested that theThe binding force between HSR and gelatin wasshould be suggested to be from hydrophobic interaction..

Key words: hydrogenated sodium rosinate, gelatin, fluorescence enhancement equation, synchronous fluorescence, resonance light scattering