Fluorescence quenching immunoassay method using unlabeled europium chelate:  Determination metallothionein (MT)

Abstract

A new nonisotopic immunoassay-fluorescence quenching immunoassay method using unlabeled europium chelate is described. The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels. The procedure is simple because labeling process is omitted.The concentration of metallothionein (MT) is inversely proportion al to fluorescent intensity. The fluorescence intensity is in linear with the concentration of MT between 0 to 1.3μg/ml. The recovery of MT in Human serum is 95% to 100%. The relative standard deviation of immunoassay is less than 10%.

Key words: SERUM, EUROPIUM COMPLEX, FLUORESCENCE ANTI-BODY ANALYSIS, METALLOTHIONEIN

CLC Number:

O657

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YAO FENGJI;ZHANG XIAOHU;CI YUNXIANG. Fluorescence quenching immunoassay method using unlabeled europium chelate: Determination metallothionein (MT)[J]. Acta Chimica Sinica, 1995, 53(10): 1015-1017.

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非标记铕配合物荧光免疫分析法研究: 测定血清中的金属硫蛋白

Fluorescence quenching immunoassay method using unlabeled europium chelate: Determination metallothionein (MT)

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