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Acta Chimica Sinica ›› 1996, Vol. 54 ›› Issue (5): 504-510. Previous Articles Next Articles
Original Articles
高莹;郑用熙
发布日期:
GAO YING;ZHENG YONGXI
Published:
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In this report, we determined the distribution of trypsin in the Aqueous Surfactant Two Phases (AS2P). The influence of SDS, C12NE and AS2P on the activity and conformation of trypsin was compared. The results showed that the denaturation efficiency of SDS is much higher than that of C12NE, while the optimal mixture of SDS and C12NE, which forms AS2P, has the least effect on trypsin. In AS2P the interaction of SDS with C12NE was strong, due to the electrostatic and the hydrophobic interactions, SDS could hardly be adsorbed by the enzyme molecule. So that, trypsin in AS2P was not changed both in activity and conformation.
Key words: ULTRAVIOLET SPECTROPHOTOMETRY, SURFACTANTS, TRYPSIN, BIOACTIVE SUBSTANCE, LAURYL SODIUM SULFATE
CLC Number:
TQ423
GAO YING;ZHENG YONGXI. The nature of aqueous surfactant two phases (AS2P) Ⅱ: The application of AS2P in biological active substance[J]. Acta Chimica Sinica, 1996, 54(5): 504-510.
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