Bioelectrochemical activities of the polyaniline xanthine oxidase electrode

Abstract

The catalytic action of the polyaniline xanthine oxidase electrode, which was formed by immobilization of xanthine oxidase on the polyaniline film using the electrochemical method, has kinetic characteristics of the typical enzyme-catalyzed reaction. The enzyme electrode has a very fast bioelectrochemical response and high stability. The apparent Michealis constant, optimum pH and activation energy for the immobilized xanthine oxidase are 21×10^-^6mol.dm^-^3, 8.4 and 85.1kJ.mol^-^1, respectively. At a very low concentration of the substrate, such as 2×10^-^6mol.dm^-^3, xanthine can be determined reliably by using a polyaniline oxidase electrode.

Key words: BIOLOGICAL ACTIVITY, XANTHINE, OXIDASE, ENZYME ELECTRODES, POLYANILINE

CLC Number:

O629

Cite this article

MU SHAOLIN;XUE HUAIGUO. Bioelectrochemical activities of the polyaniline xanthine oxidase electrode[J]. Acta Chimica Sinica, 1995, 53(6): 521-525.

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