Acta Chimica Sinica ›› 1994, Vol. 52 ›› Issue (8): 767-773. Previous Articles     Next Articles

Original Articles

热动力学对比进度法VI. 单底物酶促反应的热动力学研究

刘劲松;曾宪诚;邓郁;田安民   

  1. 四川大学化学系
  • 发布日期:1994-08-15

Reduced extent method for thermokinetics VI. thermokinetic research of the enzyme-catalyzed one-substrate reaction

LIU JINSONG;ZENG XIANCHENG;DENG YU;TIAN ANMIN   

  • Published:1994-08-15

Transformation equations for thermokinetics of the enzyme-catalyzed one-substrate reactions is deduced. Reduced extent equation for its reaction kinetics and math. model for its thermokinetics have been derived and used to calculate kinetic parameters (Km, Vm) and molar enthalpy (DH) simultaneously by analyzing a thermogram. Thermokinetics of hydrolysis of N-acetyl-L-tyrosine Et ester catalyzed by a-chymotrypsin was studied with a conduction colorimeter, and the characteristics of this method in this paper have been discussed in detail.

Key words: MATHEMATICAL MODELS, CALORIMETRY, THERMODYNAMICS, CHYMOTRYPSIN

CLC Number: