Acta Chimica Sinica ›› 2007, Vol. 65 ›› Issue (16): 1728-1732. Previous Articles     Next Articles

Reports

溶液pH和阳离子对端粒G-四链体DNA形成和结构的影响

周江, 袁谷*   

  1. (北京大学化学与分子工程学院化学生物学系 北京分子科学国家实验室 生物有机与分子工程教育部重点实验室 北京 100871)
  • 投稿日期:2007-03-29 修回日期:2007-05-24 发布日期:2007-08-28
  • 通讯作者: 袁谷

Effect of pH and Cations on the Formation and Structure of Human Telomeric G-Quadruplex DNA

ZHOU Jiang; YUAN Gu*   

  1. (Beijing National Laboratory for Molecular Sciences and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871)
  • Received:2007-03-29 Revised:2007-05-24 Published:2007-08-28
  • Contact: YUAN Gu

Electrospray ionization mass spectrometry and circular dichroism were used to investigate the factors which affect the motif of the human telomeric quadruplex, such as pH and cations. ESI-MS and CD spectra indicated that pH could induce both the conformational transition and the dissociation of the quadruplex. Moreover, Kand NH4 cations play an important role in the formation of the quadruplex.

Key words: telomere, G-quadruplex DNA, ESI mass spectrometry, circular dichroism