To establish a new screening method for xanthine oxidase inhibitor using screen printed electrode the one-off screen printed carbon paste electrode was prepared using screen printing technology. Carboxylic multi-walled carbon nanotubes were absorbed on the working surface of the screen printed electrode. This is a simple and rapid amperometry analysis method of uric acid determination which was applied to xanthine oxidase inhibitor screening in vitro. A sensitive response was obtained at an applied potential of 0.3 V vs. Ag/AgCl. The response time is 30 s. A linear response to uric acid was obtained up to 300 μmol• L^－1 with a limit of detection of 1 μmol•L^－1. The linear regression equation is Y＝43.8240X－0.1592 (r＝0.9998). The analysis was applied to four kinds of water extracts of traditional Chinese medicine. The group of Laranthus tanakae with a concentration of 500 μg•mL^－1 is significantly different from the control group. The method is simple and rapid with low manufacturing costs of the electrode and a simple apparatus.

Key words: screen printed electrode, xanthine oxidase inhibitor, drug screening, amperometry