Preliminary Searching for Extraction Technology and Bioactive Parts of Radix Isatidis Based on Microcalorimetry

Acta Chimica Sinica ›› 2008, Vol. 66 ›› Issue (9): 1111-1116. Previous Articles Next Articles

Original Articles

基于微量热法的板蓝根提取方法和活性部位挑选

孔维军¹,2，赵艳玲*^,1，山丽梅¹，肖小河¹，郭伟英²

(¹解放军302医院全军中药研究所北京 100039)
(²辽宁医学院药学院锦州 121001)

投稿日期:2007-11-07 修回日期:2007-11-27 发布日期:2008-05-14
通讯作者: 赵艳玲

Preliminary Searching for Extraction Technology and Bioactive Parts of Radix Isatidis Based on Microcalorimetry

KONG Wei-Jun¹² ZHAO Yan-Ling*¹ SHAN Li-Mei¹ XIAO Xiao-He¹ GUO Wei-Ying²

(¹PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039)
(²Pharmacy College, Liaoning Medical University, Jinzhou 121001)

Received:2007-11-07 Revised:2007-11-27 Published:2008-05-14
Contact: ZHAO Yan-Ling

Abstract

Using a TAM air microcalorimetric system, the influence of different extractions from Radix Isatidis on Escherichia coli growth was investigated at 37 ℃ by microcalorimetry. In accordance with the thermogenic curves and parameters, the potential pharmacological action of decoction and its five bioactive parts was analyzed from the point of view of traditional Chinese medicinal (TCM) theory to screen the best extraction technology and the strongest antibacterial parts of Radix Isatidis. Meanwhile, the minimum inhibitory concentration of the strongest antibacterial parts of Radix Isatidis against Escherichia coli growth was obtained by a tube dilution method. The results show that microcalorimetry can be used to screen the extraction technology and bioactive parts of Radix Isatidis, furthermore to provide some suggestions for the study of TCM.

Key words: Radix Isatidis, microcalorimetry, Escherichia coli, extraction technology, bioactive part

Cite this article

KONG Wei-Jun¹² ZHAO Yan-Ling*¹ SHAN Li-Mei XIAO Xiao-He GUO Wei-Ying². Preliminary Searching for Extraction Technology and Bioactive Parts of Radix Isatidis Based on Microcalorimetry[J]. Acta Chimica Sinica, 2008, 66(9): 1111-1116.

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基于微量热法的板蓝根提取方法和活性部位挑选

Preliminary Searching for Extraction Technology and Bioactive Parts of Radix Isatidis Based on Microcalorimetry

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