The synthesis and properties of β-cyclodextrin derivatized pancreatin used as chiral selector were investigated in this paper. Such β-cyclodextrin derivatized pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether in boric acid buffer at pH value 8.7. Amino group was coated on the internal surface of the silica capillary by sol-gel technology with triethoxylmethylsiloxane and (3-aminopropyl)trimethoxysiloxane. Covalent binding of β-cyclodextrin derivatized pancreatin was performed by glutaraldehyde. Tryptophan, chlorpheniramine, ibuprofen, promethazine and atroopine were baseline separated by β-cyclodextrin derivatized pancreatin in pressure capillary electrochromatography. High resolution and large relative retention time were obtained at high operation voltage in the separation of chlorpheniramine.

Key words: β-cyclodextrin, pancreatin, chiral separation, capillary electrochromatography