A new method combined with immunity, electroporation and nano-TiO₂ photo-catalysis was successfully used to kill LoVo cancer cells. At first monoclonal antibody was adsorbed on the surface of nano-TiO₂ particles, then the antibody-nano-TiO₂ conjugates would adsorb on the membrane of LoVo cells spontaneously. Electroporation method was used to produce micro holes on the membrane of LoVo cells, which promoted the entering of antibody-nano-TiO₂ conjugates into the cancer cells. Under the irradiation of ultra violet light, the cancer cells could be killed based on the photocatalysis effect of nano-TiO₂ particles within cells. The results show that the combined technique is very efficient for killing cancer cells. In the case of using low concentration of antibody-nano-TiO₂ as 3.12 μg/mL, and under the irradiation of ultra violet light at 4 mW/cm², all of the LoVo cancer cells could be killed within 30 min. The photo-killing effi-ciency for LoVo cancer cells was much higher than that for human normal TE353.sk skin cells, indicating the high selectivity of this method. This novel combination method might be used as a new technique for the cancer treatment in the future.

Key words: human colon LoVo cell, TE353.sk cell, nano-TiO₂, immunity cell electroporation, photo-ca- talysis, treatment of cancer