The separation and detection of urinary proteins have been conducted by microchip-based capillary electrophoresis (MCE), and application foreground of MCE in clinical diagnosis of various states of disease was discussed. Microchip-based capillary electrophoresis of urinary proteins could offer several advantages over standard urinary protein assay methods such as SDS-PAGE and conventional capillary electrophoresis, including the decrease of sample consumption and analysis time. The microchip used in these studies was designed to realize on-chip separation of urinary proteins with UV detection. The characteristic absorbency near 210 nm of proteins was adopted under the condition: the electrophoretic buffer at 75 mmol•L^－1 borate with 0.5 mol•L^－1 lactate at pH 10.5. Urine samples from four clinical cases were selected as target for microchip-based urinary protein assays, and the results were consistent with those of commercial Helena Rapid Electrophoreses system.

Key words: capillary electrophoresis microchip, UV detection, clinical diagnosis, urinary protein