The fluorescent properties of pyrene monomer in various environments, such as β-CD, CTAB, SDS and glycerl solutions, have first been studied by three-dimensional fluorescence spectrometry. The results show that the F~3/F~4 ratio, namely the relative intensity of peak 3 (λ~e~x/λ~e~m=340/378nnm) to peak 4 (λ~e~x/λ~e~m=340/396nm), can be used to estimate the formation of organized medium, but not the polarity of environment. Only F~1/F~3 ratio, the relative intensity of peak 1 (λ~e~x/λ~e~m=275/378nm) to peak 3, can be used as the probe for the polarity of environment. The three-dimensional fluorescence spectra of pyrene have superiority in studying the properties of environment.

Key words: MICELLE, FLUORESCENCE CHARACTERISTIC, PYRENE, FLUORESCENCE PROBE