Acta Chimica Sinica ›› 1997, Vol. 55 ›› Issue (10): 961-966. Previous Articles     Next Articles

Original Articles

细胞动力学研究VI. 厌氧菌生长过程热化学特征

刘义;谭安民;谢昌礼;屈松生;赵必均   

  1. 武汉大学化学系;广州军区武汉总医院
  • 发布日期:1997-10-15

Studies on cell kinetics VI. Thermochemical characteristics of the growth of anaerobes

LIU YI;TAN ANMIN;XIE CHANGLI;QU SONGSHENG;ZHAO BIJUN   

  • Published:1997-10-15

By using LKB2277 Bioactivity Monitor, we have determined the thermogenetic power curves of three kinds of Anaerobes: Bacteroides distasonis, Clostridium difficille, Peptococcus niger. Thermokinetic equation, ln[P/(Pm-P)^v^+^1]=ln[P0/(Pm-P0)^v^+^1]+k.t, was established based on the generalized logistic equation. The rate constants (k) of cell growth were obained, and for BActeroids distasonis, the activation properties, such as activation energy, the entropy of activation, Gibbs function of activation and equilibrium constant of activation were also calculated. This thermokinetic equation is very suitable for cell growth of separated culture. It was compared with exponential and logistic model. It is very significant for the study of bacterial limited growth and their characteristics.

Key words: CALORIMETRY, ACTIVATION ENERGY, TRANSITION STATE THEORY, ANAEROBIC BACTERIA

CLC Number: