Acta Chimica Sinica ›› 2000, Vol. 58 ›› Issue (11): 1452-1456. Previous Articles     Next Articles

Original Articles

电喷雾/飞行时间质谱法测定红霉素类抗生素的准 确质量

胡守刚;郭寅龙;吕龙   

  1. 中国科学院上海有机化学研究所.上海(200032)
  • 发布日期:2000-11-15

Determination of the accurate mass of erythromycin analogue antibiotic by electrospray ionization/time-of-flight mass spectrometry

Hu Shougang;Guo Yinlong;Lu Long   

  1. Shanghai Inst Organ Chem., CAS.Shanghai(200032)
  • Published:2000-11-15

Using polyethylene glycol (PEG) as internal reference, electrospray ionization (ESI)/time-of-flight mass spectrometry (TOF-MS) was developed to determine the accurate mass of the protonated molecular ions of five erythromycin analogue antibiotics. Compared with the theoretical values, all relative errors were less than 5×10^-^6. PEG can form three adduct ions with K^+, Na^+, H^+, respectively, and may appear with single kind of adduct ions in suitable experiment conditions. PEG was recommended as a general internal reference compound. Besides, two-peak calibration is preferred to the multi- peak calibration. Scan mass width and scan speed were also studied, 104~144amu 5 seconds was recommedned. Using polyethylene glycol(PEG) as internal reference, ESI/TOF mass spectrometry can determine the accurate mass of the protonated molecular ions of the unsteady basic compounds. This method is simple and expeditious.

Key words: TIME-OFF FLIGHT MASS SPECTROMETRY, ERYTHROMYCINUM, ANTIBIOTICS

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