Microcalorimetric Study on the Growth of Escherichia coli HB101 Effected by Recombined Plasmid

Abstract

The growth metabolism of Pseudomonas maltophilia (P. maltophilia) AT18, Escherichia coli HB101, E. coli HB101/WSY (containing mel gene) and E. coli HB101/pUC18 was studied by means of microcalorimetry. The experimental results show that bacterial growth metabolism is correlated with the sizes of plasmid, and low temperature treatment has obvious effect on the growth of engineering bacteria E. coli HB101/WSY.

Key words: microcalorimetry, recombined plasmid, mel gene, gene engineering bacteria

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LIU Yi*^,1,WANG Ge-Ling²,ZHAO Ru-Ming^{a. Microcalorimetric Study on the Growth of Escherichia coli HB101 Effected by Recombined Plasmid[J]. Acta Chimica Sinica, 2005, 63(4): 327-331.}

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重组质粒对大肠杆菌HB101生长影响的微量热研究

Microcalorimetric Study on the Growth of Escherichia coli HB101 Effected by Recombined Plasmid

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[1]	Li Yuesheng, Li Benqiang, Sun Shaofa. Microcalorimetric and Microscopic Studies on the Antibacterial Activities of CdTe(QDs)/TiO₂ Nanocomposites and the Mechanism of Cell Damage on Escherichia coli [J]. Acta Chimica Sinica, 2013, 71(12): 1656-1662.
[2]	Huang Xu, Han Yuchun, Wang Yilin. Thermodynamic Investigation on Micellization of Cationic Gemini Surfactants with Nitrophenoxy Groups in Hydrophobic Chains [J]. Acta Chimica Sinica, 2013, 71(06): 897-905.
[3]	Fan Gaochao, Huang Zaiyin, Chen Jie, Ma Yujie. Impact of Size Effects on the Thermodynamic Properties of Zinc Oxide Micro/nano System [J]. Acta Chimica Sinica, 2012, 70(07): 938-942.
[4]	WANG Lu-De, WANG Teng-Hui, GUO Yun-Xiao, HUANG Zai-Yin. Preparation and Standard Molar Enthalpy of Formation for the Octahedron BaMoO₄ Nanostructures [J]. Acta Chimica Sinica, 2011, 69(21): 2637-2640.
[5]	YANG Chu-Juan, KONG Chang-Jing, ZHANG Ge, BI Gong-Yan, LI Jing, ZHANG Hong-Lin. Study on Enzymatic Degradation of Cellulose in Diisooctyl Succinate Sodium Sulfonate (AOT)/Triton X-100 Mixed Reverse Micellar System [J]. Acta Chimica Sinica, 2010, 68(9): 839-844.
[6]	ZHANG Shao-Feng, YAN Dan, TANG Hui-Ying, FENG Xue, HAN Yu-Mei, YANG Ming, WANG Yue-Sheng, XIAO Xiao-He. Microcalorimetric Investigation on Physiological Activity of Red Blood Cells Affected by Excipient of Polysorbate 80 [J]. Acta Chimica Sinica, 2010, 68(20): 2119-2124.
[7]	DAI Chun-Mei, WANG Ga-Ba, KONG Wei-Jun, XIAO Xiao-He, BANG Cheng, DIAO Yan-Ling, JIN Cheng. Investigation on the Antibacterial Activity of Coptis chinensis Franch and Its Components Compatibility by Microcalorimetry [J]. Acta Chimica Sinica, 2010, 68(10): 936-940.
[8]	TANG Hui-Yang, HAN Yu-Mei, YAN Dan, ZHANG Shao-Feng, LUO Yun, XIAO Xiao-He, LIU Rong-Hua, BANG Cheng. Microcalorimetric Investigation of Toxic Effects of Diester Diterpenoid Alkaloids from Aconiti Class on Tetrahymena thermophila BF5 Growth [J]. Acta Chimica Sinica, 2010, 68(03): 205-210.
[9]	JUE Chi-Qing, LIN Rui-Sen, FANG Wen-Jun. Disturbance of Environmental Atmosphere on the Growth Rate Constants of E. coli Determined by Microcalorimetry Ampoule Method [J]. Acta Chimica Sinica, 2010, 68(01): 50-54.
[10]	JUE Chi-Qing, LIN Rui-Sen, FANG Wen-Jun. Microcalorimetry investigation on active position of Vitex negundo Linn against E. coli [J]. Acta Chimica Sinica, 2009, 67(23): 2692-2696.
[11]	KONG Wei-Jun, DIAO Yan-Ling, WANG Ga-Ba, XIAO Xiao-He. Investigation on the Anti-Candida albicans Effect of Palmatine Hydrochloride Based on Microcalorimetry and Principal Component Analysis [J]. Acta Chimica Sinica, 2009, 67(21): 2511-2516.
[12]	KONG Wei-Jun¹² ZHAO Yan-Ling¹ SHAN Li-Mei XIAO Xiao-He GUO Wei-Ying². Preliminary Searching for Extraction Technology and Bioactive Parts of Radix Isatidis* Based on Microcalorimetry [J]. Acta Chimica Sinica, 2008, 66(9): 1111-1116.
[13]	LI Xiang-Rong*^,1,2 ,GUO Wei² ,LU Yan. Denaturation Study of Bovine Serum Albumin Induced by the Guanidine Chloride or Urea by Microcalorimetry [J]. Acta Chimica Sinica, 2008, 66(5): 515-519.
[14]	. Thermokinetic Studies of Action of Complexes RE(Hsal)2•(tch)•2H2O on Growth Metabolism of Escherichia. Coli [J]. Acta Chimica Sinica, 2008, 66(24): 2686-2692.
[15]	GAO Gui-Lan^1;FU Shi-Lin²; KONG Zhe; WANG Jian-Tao; ZHANG Hong-Lin*^,1. Study on the Oscillating Systems of Siwu Decoction and Its Single Herbs by Microcalorimetry [J]. Acta Chimica Sinica, 2007, 65(18): 1970-1974.