Study on the interaction mechanism between mitomycin C  and DNA by fluorescence method

Acta Chimica Sinica ›› 1999, Vol. 57 ›› Issue (2): 171-175. Previous Articles Next Articles

Original Articles

荧光法研究丝裂霉素C与DNA的作用机制

王瑞琼;鄢远;黄坚锋

南昌大学化学系应用化学研究所

发布日期:1999-02-15

Study on the interaction mechanism between mitomycin C and DNA by fluorescence method

Wang Ruiqiong;Yan Yuan;Huang Jianfeng

Published:1999-02-15

Abstract

In this paper, the interaction mechanism between mitomycin C and calf thymus DNA has been studied by fluorescence method using EB as an extrinsic fluorescence probe. The results show that mitomycin C can interact with DNA only after being reductively activated by Na2S2O4, and the interaction mode between them should be minor groove binding and partial intercalation. This result further proves that mitomycin C interacts with DNA in the mode of cross-linking.

Key words: DEOXYRIBONUCLEIC ACID, MITOMYCIN, ETHIDIUM BROMIDE

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Cite this article

Wang Ruiqiong;Yan Yuan;Huang Jianfeng. Study on the interaction mechanism between mitomycin C and DNA by fluorescence method[J]. Acta Chimica Sinica, 1999, 57(2): 171-175.

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荧光法研究丝裂霉素C与DNA的作用机制

Study on the interaction mechanism between mitomycin C and DNA by fluorescence method

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