Studies on Fingerprint of Epimedium koreanum in Changbai Mountains by High-performance Liquid Chromatography

Acta Chimica Sinica ›› 2009, Vol. 67 ›› Issue (22): 2613-2618. Previous Articles Next Articles

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朝鲜淫羊藿的色谱指纹图谱及液相色谱/电喷雾串联质谱分析

窦建鹏^a,b，宋凤瑞^a，刘志强^a，刘淑莹^*,a

(^a中国科学院长春应用化学研究所长春 130022) (^b吉林大学生物与农业工程学院长春 130022)

投稿日期:2009-01-01 修回日期:2009-03-24 发布日期:2009-07-08
通讯作者: 刘志强 E-mail:liuzq@ciac.jl.cn

Studies on Fingerprint of Epimedium koreanum in Changbai Mountains by High-performance Liquid Chromatography

Dou, Jianpeng ^a,b，Song, Fengrui^a，Liu, Zhiqiang^a，Liu, Shuying^*,a

(^aChangchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022) (^b College of Biological and Agricultural Engineering, Jilin University, Changchun 130022)

Received:2009-01-01 Revised:2009-03-24 Published:2009-07-08

Abstract

A high-performance liquid chromatography (HPLC) fingerprint method was developed for analysis of Epimedium koreanum Nakai in Changbai mountains. The chromatographic conditions are as follows: Zorbax Extend C18 (5 µm, 4.6 mm×250 mm, Agilent) column; mobile phase: 0.5% acetic acid-water and methanol, gradient elute; flow rate: 0.6 mL/min; column temperature: 25 ℃; wavelength: 270 nm; run time: 80 min. 13 common peaks were selected from 18 crude extracts of Epimedium koreanum, and their structures were identificated by electrospray ionization tandem mass spectrometry (ESI-MS/MS), and according to the data of MS, the structures of the 13 compounds were speculated. Relative standard deviation values (RSD) of precision, stability and repeatability are less than 3.0%. The results indicate the HPLC fingerprint of Epimedium koreanum Nakai. will show more characters through identification of component structures using an HPLC-MS/MS method.

Key words: Epimedium koreanum, flavonoid, high-performance liquid chromatography, electrospray ionization mass spectrometry, fingerprint spectrum

Cite this article

DOU Jian-Feng, SONG Feng-Rui, LIU Zhi-Jiang, LIU Chu-Ying. Studies on Fingerprint of Epimedium koreanum in Changbai Mountains by High-performance Liquid Chromatography[J]. Acta Chimica Sinica, 2009, 67(22): 2613-2618.

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朝鲜淫羊藿的色谱指纹图谱及液相色谱/电喷雾串联质谱分析

Studies on Fingerprint of Epimedium koreanum in Changbai Mountains by High-performance Liquid Chromatography

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