Investigation on the Competition Interaction of Ferulic Acid and Paeonol with Human Serum Albumin by High-performance Affinity Chromatography

Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (01): 55-61. Previous Articles Next Articles

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中药小分子阿魏酸和丹皮酚与人血清白蛋白的竞争结合作用研究

雷根虎¹，刘丽亭¹，戴小军¹^{,2，卫引茂*^{,1，龚波林*^,2}}

(¹西北大学合成与天然功能分子化学教育部重点实验室化学与材料科学学院西安 710069)
(²宁夏大学能源化工重点实验室银川 750021)

投稿日期:2009-04-10 修回日期:2009-07-26 发布日期:2010-01-20
通讯作者: 卫引茂 E-mail:ymwei@nwu.edu.cn

Investigation on the Competition Interaction of Ferulic Acid and Paeonol with Human Serum Albumin by High-performance Affinity Chromatography

Lei Genhu¹ Liu Liting¹ Dai Xiaojun¹^{,2 Wei Yinmao*^{,1 Gong Bolin*^,2}}

(¹Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry & Material Science, Northwest University, Xi'an 710069)
(² Key Laboratory of Energy & Chemical Engineering, Ningxia University, Yinchuan 750021)

Received:2009-04-10 Revised:2009-07-26 Published:2010-01-20

Abstract

The competition interaction of two active components of Chinese traditional herb, ferulic acid (FA) and paeonol (PAE), with immobilized human serum albumin (HSA) on chromatographic support under human physiological condition (pH 7.4) has been investigated by high-performance affinity chromatography (HPAC). Based upon the assumption that there were several independent types of binding sites on an HSA molecule for each drug, the interaction of immobilized HSA with FA and PAE was investigated by frontal analysis and competition displacement analysis. The results showed that there was only a single binding site in the HSA molecule for FA and PAE. As FA and PAE coexisted, they had a direct competition for the indole site (site II) of HSA molecule. According to the thermodynamic parameters, the main force between HSA and FA or PAE was deduced to be hydrogen bonding. The conclusion that both PAE and FA competed with each other for the same binding site of HSA molecule could be used to explain some phenomena in the Chinese medicine clinic, in which the clinical efficacy of each drug can be improved by utilizing the compatibility of Chinese traditional medicine.

Key words: high-performance affinity chromatography, human serum album, ferulic acid, paeonol, small molecular-biopolymer interaction

Cite this article

LEI Gen-Hu, LIU Li-Ting, DAI Xiao-Jun, WEI Yin-Mao, GONG Bei-Lin. Investigation on the Competition Interaction of Ferulic Acid and Paeonol with Human Serum Albumin by High-performance Affinity Chromatography[J]. Acta Chimica Sinica, 2010, 68(01): 55-61.

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中药小分子阿魏酸和丹皮酚与人血清白蛋白的竞争结合作用研究

Investigation on the Competition Interaction of Ferulic Acid and Paeonol with Human Serum Albumin by High-performance Affinity Chromatography

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