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Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (02): 162-168. Previous Articles     Next Articles

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板式磁颗粒化学发光免疫分析测定人血清中糖类抗原125

李智勇1,2，肖勤1，应希堂3，李振甲3，林金明*,1   

  1. (1清华大学化学系生物有机磷教育部重点实验室 北京 100084)
    (2中国科学院生态环境研究中心 北京 100085)
    (3北京科美东雅生物技术有限公司 北京 100094)
  • 投稿日期:2009-06-01 修回日期:2009-07-23 发布日期:2010-03-17
  • 通讯作者: 林金明 E-mail:jmlin@mail.tsinghua.edu.cn,jmlin@mail.rcees.ac.cn

Micro-plate Magnetic Chemiluminescence Immunoassay of Carbohydrate Antigen 125 in Serum

Li Zhiyong1,2 Xiao Qin1 Ying Xitang3 Li Zhenjia3 Lin Jinming*,1   

  1. (1 Educational Ministry Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084)
    (2 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085)
    (3 Beijing Chemclin Biotech Co. Ltd., Beijing Academy of Science and Technology, Beijing 100094)
  • Received:2009-06-01 Revised:2009-07-23 Published:2010-03-17

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A micro-plate magnetic chemiluminescence immunoassay (MMCLIA) for the determination of human carbohydrate antigen 125 (CA125) was developed based on traditional micro-plate chemiluminescence immunoassay (micro-plate CLIA) and magnetic particle-based chemiluminescence immunoassay (MP-CLIA). This method used magnetic particles as solid support and the micro-plate as a reactor. The horseradish peroxidase (HRP)-luminol-H2O2 chemiluminescent system was chosen as the detection system. The results showed that the developed method had a detection limit of 2.0 U/mL with a linear range of 0～400 U/mL. The developed method showed wider linear range than the micro-plate CLIA. The sensitivity and precision were higher than the commonly used MP-CLIA method. In addition, the method has been successfully applied to detection of CA125 in human serum with good correlation compared with the Roche method.

Key words: chemiluminescence immunoassay, magnetic particle, micro-plate, carcinoembryonic antigen 125