Acta Chimica Sinica ›› 2010, Vol. 68 ›› Issue (21): 2151-2155. Previous Articles     Next Articles

Full Papers

三组分抗原的磁分离及分离效率的SERS研究

陈帅,姚建林*,韩三阳,顾仁敖*   

  1. (苏州大学材料与化学化工学部 苏州 215123)
  • 投稿日期:2010-04-18 修回日期:2010-05-10 发布日期:2010-07-12
  • 通讯作者: 顾仁敖 E-mail:ragu@suda.edu.cn
  • 基金资助:

    双金属核壳结构纳米粒子的制备及其表面增强拉曼光谱研究;基于表面增强拉曼光谱和电化学的痕量重金属离子检测; 过渡金属核壳结构纳米颗粒的表面增强拉曼光谱研究及其应用;新型核壳金属纳米材料的制备、表征及其电催化研究

Magnetic Separation of Tri-component Antigens and SERS-Based Evaluation

CHEN Shuai, YAO Jian-Lin, HAN San-Yang, GU Ren-Ao   

  1. (College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123)
  • Received:2010-04-18 Revised:2010-05-10 Published:2010-07-12

Magnetic γ-Fe2O3@Au core/shell nanoparticles were prepared by the growth of Au shell onto the seeds of γ-Fe2O3 nanoparticles. The γ-Fe2O3@Au nanoparticles were functionalized by the modification of antibody. By the specific interaction between antigen and antibody, one of the antigens was separated in order or two antigens were separated together with the assistance of external magnetic field. The efficiency of magnetic separation was evaluated based on surface enhanced Raman spectroscopy (SERS) immunoassay technique. The concentration limitation for the magnetic separation and the SERS evaluation were obtained. The results showed that the method of magnetic immune separation has been achieved to separate any components in the media with three antigens without any influence on the existence of other antigens. The concentration of the separated antigen was reduced to the detection limitation of SERS-based immunoassay which could reach about 0.1 pg/mL.

Key words: magnetic core-shell nanoparticles, immunoassay, surface-enhanced Raman spectroscopy (SERS), magnetic separation, separation limitation