Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (07): 803-809. Previous Articles     Next Articles

Full Papers

LC-MS/MS法快速高效检测尿液中尼古丁及其9种代谢物

周宛虹,王晔,余苓,金永明,郑赛晶*   

  1. (上海烟草集团有限责任公司 上海 200082)
  • 投稿日期:2003-08-27 修回日期:2010-10-13 发布日期:2010-12-09
  • 通讯作者: 郑赛晶 E-mail:zhengsj@sh.tobacco.com.cn

A Rapid LC-MS/MS Method for Determination of Nicotine and Its 9 Metabolites in Urine

ZHOU Wan-Hong, WANG Ye, YU Ling, JIN Yong-Ming, ZHENG Sai-Jing   

  1. (Shanghai Tobacco Group Co., Ltd., Shanghai 200082)
  • Received:2003-08-27 Revised:2010-10-13 Published:2010-12-09
  • Contact: Sai-Jing ZHENG E-mail:zhengsj@sh.tobacco.com.cn

A fast, highly efficient method for the detection of nicotine and its nine metabolites in urine was established in this paper. The quantification was achieved by using d3-nicotine, d3-cotinine, d3-3-OH-cotinine, d3-nicotine glucuronic acid, d3-cotinine glucuronic acid and d3-3-OH-cotinine glucuronic acid as internal standards under atmosphere pressure chemical ionization (APCI) condition of LC-MS/MS. Nineteen samples were quantified and then analyzed by discriminant analysis (DA). The pretreatment of this method was simple, time-saving and all compounds were eluted within 3.8 min. The relative standard deviation (RSD%) of nicotine and its nine metabolites were between 0.5% and 5.5% with the recovery rate ranged from 94% to 109%. The linear correlations were no less than 0.995. Smokers of different levels were significantly distinguished using DA and a strong positive correlation was found between the value of nicotine and its 9 metabolites in 24 h urine and smoke amount.

Key words: LC-MS/MS, rapid determination, urine, nicotine, metabolite, discriminant analysis