Study on the Interaction between Magnetic Iron Oxide Nanoparticles with Calf Thymus DNA

Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (16): 1915-1919. Previous Articles Next Articles

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磁性纳米氧化铁与小牛胸腺DNA相互作用研究

杨芹芹，郭强，梁建功，韩鹤友*

(华中农业大学理学院化学生物学研究所农业微生物学国家重点实验室武汉 430070)

投稿日期:2010-12-23 修回日期:2011-05-18 发布日期:2011-04-27
通讯作者: 韩鹤友 E-mail:hyhan@mail.hzau.edu.cn
基金资助:
近红外量子点成像用于Bt毒素受体确认及Bt杀虫机理研究;转基因动物抽样及快速、高通量检测技术体系研究;从分子及细胞水平研究量子点的毒理效应

Study on the Interaction between Magnetic Iron Oxide Nanoparticles with Calf Thymus DNA

YANG Qin-Qin, GUO Qiang, LIANG Jian-Gong, HAN He-You

(State Key Laboratory of Agricultural Microbiology, Institute of Chemical Biology, College of Science, Huazhong Agricultural University, Wuhan 430070)

Received:2010-12-23 Revised:2011-05-18 Published:2011-04-27

Magnetic iron oxide nanoparticles (MNPs) were synthesized by microwave-assisted coprecipitation. The effects of MNPs on the calf thymus DNA (ct DNA) were investigated by UV-vis spectra, Raman spectra and Agarose gel electrophoresis, respectively. UV-vis spectra revealed the hyperchromic effect on ct DNA due to the exposure of basic group that induced by MNPs. Raman spectra indicated that the basic group, ribodesose and backbone were disturbed in the presence of MNPs. However, Agarose gel electrophoresis confirmed that DNA melting or DNA strand break have not occurred under the influence of MNPs. The research had great significance for the safety application of MNPs in bio-medical field.

Key words: magnetic iron oxide nanoparticles, calf thymus DNA, microwave-assisted, hyperchromic effect, Raman spectra

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YANG Qin-Qin, GUO Qiang, LIANG Jian-Gong, HAN He-You. Study on the Interaction between Magnetic Iron Oxide Nanoparticles with Calf Thymus DNA[J]. Acta Chimica Sinica, 2011, 69(16): 1915-1919.

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磁性纳米氧化铁与小牛胸腺DNA相互作用研究

Study on the Interaction between Magnetic Iron Oxide Nanoparticles with Calf Thymus DNA

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