Regularity of Toxic Alkaloids during the Combination of Veratrum nigrum and Salvia miltiorrhiza by UPLC-Q-TOF/MS

doi:10.6023/A12080606

Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (21): 2257-2264.DOI: 10.6023/A12080606 Previous Articles Next Articles

Article

UPLC-Q-TOF/MS分析不同比例丹参配伍藜芦毒性生物碱变化规律

李飞^a,b, 王宇光^b, 杨亮^b, 朱冠秀^a,b, 梁乾德^b, 马增春^b, 肖成荣^b, 谭洪玲^b, 汤响林^b, 高月^b

a 中南大学湘雅医学院长沙 570228;
b 军事医学科学院放射与辐射医学研究所北京 100850

投稿日期:2012-08-30 发布日期:2012-09-22
通讯作者: 王宇光, 高月 E-mail:gaoyue@nic.bmi.ac.cn; wang_yuguang@139.com
基金资助:
项目受国家重点基础研究发展计划(Nos. 2011CB505304, 2012CB518402)、国家自然科学基金(No. 81073149)和北京市自然科学基金(No. 7112110)资助.

Regularity of Toxic Alkaloids during the Combination of Veratrum nigrum and Salvia miltiorrhiza by UPLC-Q-TOF/MS

Li Fei^a,b, Wang Yuguang^b, Yang Liang^b, Zhu Guanxiu^a,b, Liang Qiande^b, Ma Zengchun^b, Xiao Chengrong^b, Tan Hongling^b, Tang Xianglin^b, Gao Yue^b

a Xiangya Medical College Central Sourth University, Changsha 570228;
b Beijing Institute of Radiation Medicine, Beijing 100850

Received:2012-08-30 Published:2012-09-22
Supported by:
Project supported by National Basic Research Program of China (Nos. 2011CB505304, 2012CB518402), the National Natural Science Foundation of China (No. 81073149) and the Natural Science Foundation of Beijing (No. 7112110).

1. Regularity of Toxic Alkaloids during the Combination of Veratrum nigrum and Salvia miltiorrhiza by UPLC-Q-TOF/MS.PDF(2478KB)

Abstract

In traditional chinese medicine, the combination of veratrum nigrum and salvia miltiorrhiza is traditional incompatibility. In order to investigate the intrinsic relevance of acute toxicity experiment and main toxic alkaloids variation in different ratio, ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was used to reveal the chemical profile of decoctions of veratrum nigrum and salvia miltiorrhiza in different ratio (10∶0, 10∶1, 5∶1, 2∶1, 1∶1, 1∶2, 1∶5, 1∶10). The analyses were performed on a reversed-phase C₁₈ column using a binary eluent under gradient conditions. At the same time, the trend of toxicity diversity between the veratrum nigrum and salvia miltiorrhiza was explored through acute toxicity experiment of mice. Seventeen alkaloids of veratrum nigrum can be detected, The t_R-m/z pairs and mean mass can be obtained by selected ion chromatogram, after extracted by Markerlynx XS, the dataset were subjected to principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA) to holistically compare the difference between samples, and variant changes of veratrum nigrum were observed. After fixed the dosage of veratrum nigrum as LD₅₀, when the ratio of veratrum nigrum was greater than salvia miltiorrhiza, the dissolution of some alkaloids was greater than or equal to the single decoction group of veratrum nigrum, such as verdine, pseudojervine, zygacine, rubijervine, neogermidine, germidine, 3-angeloylzygadenine and so on, with the increase proportion of salvia miltiorrhiza, these alkaloids dissolution showed a declining trend. When the ratio of veratrum nigrum was less than salvia miltiorrhiza, the dissolution of the alkaloids was less than the single decoction group of veratrum nigrum. The result of acute toxicity experiment is matched with the trend of these alkaloids, which showed us these alkaloids may be the main chemical markers of enhanced toxicity during the combination of veratrum nigrum and salvia miltiorrhiza in different ratio.

Key words: eighteen incompatible pairs, salvia miltiorrhiza, veratrum nigrum, acute toxicity experiment, ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF/MS)

Cite this article

Li Fei, Wang Yuguang, Yang Liang, Zhu Guanxiu, Liang Qiande, Ma Zengchun, Xiao Chengrong, Tan Hongling, Tang Xianglin, Gao Yue. Regularity of Toxic Alkaloids during the Combination of Veratrum nigrum and Salvia miltiorrhiza by UPLC-Q-TOF/MS[J]. Acta Chimica Sinica, 2012, 70(21): 2257-2264.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

share this article

References

[1] Xu, D.-H.; Xu, Y.-H. World Phytomed. 2002, 17, 185. (徐墩海, 徐雅红, 国外医药·植物药分册, 2002, 17, 185.)

[2] Wen, W.-F. M.S. Thesis, Dalian University of Technology, Dalian, 2006. (温伟峰, 硕士论文, 大连理工大学, 大连, 2006.)

[3] Liu, Y.-L.; Liu, G.-T. Acta Pharm. Sin. 2002, 37, 81. (刘颖琳, 刘耕陶, 药学学报, 2002, 37, 81.)

[4] Zhao, X.-F.; Wang, Z.-Q.; Zhao, X.; Zheng, X.-H. Chin. Tradit. Pat. Med. 2005, 27, 1068. (赵新锋, 王中秋, 赵欣, 郑晓晖, 中成药, 2005, 27, 1068.)

[5] Li, Y.-J.; Sun, Z.-L. J. Practical Med. Tech. 2006, 13, 2903. (李以菊, 孙泽玲, 实用医技杂志, 2006, 13, 2903.)

[6] Peng, Z.-H.; Yuan, L.-M.; Han, M.-L. J. Chin. Med. Mater. 2001, 24, 690. (彭招华, 袁侣明, 韩民利, 中药材, 2001, 24, 690.)

[7] Zhou, C.-X.; Luo, H.-W.; Dan, Y.-Z.-W. J. Chin. Pharm. Univ. 1999, 30, 411. (周长新, 罗厚蔚, 丹羽正武, 中国药科大学学报, 1999, 30, 411.)

[8] Pan, L.-S.; Lü, X.-Y.; Wu, P.-D. Guiding J. TCM, 2005, 11, 3. (潘浪胜, 吕秀阳, 吴平东, 中医药导报, 2005, 11, 3.)

[9] Xia, J.-M.; Meng, X.-S.; Zhang, Y.; Zhang, M. Spectrosc. Spect. Anal. 2008, 28, 465. (夏锦明, 孟宪生, 张颖, 张敏, 光谱学与光谱分析, 2008, 28, 465.)

[10] Zhou, S.-S.; Ma, Z.-C.; Liang, Q.-D.; Wang, Y.-G.; Tan, H.-L.; Xiao, C.-R.; Zhang, B.-L.; Gao, Y. Acta Chim. Sinica 2012, 70, 284. (周思思, 马增春, 梁乾德, 王宇光, 谭洪玲, 肖成荣, 张伯礼, 高月, 化学学报, 2012, 70, 284.)

[11] Tanaka, N.; Suto, S.; Kobayashi, J. Chem. Pharm. Bull. 2011, 59, 909.

[12] Cong, Y.; Zhou, Y.-B.; Chen, J.; Zeng, Y.-M.; Wang, J.-H. J. Pharm. Biomed. Anal. 2008, 48, 573.

[13] Cong, Y.; Wang, J.-H.; Wang, R.; Zeng, Y.-M.; Liu, C.-D.; Li, X. J. Asian Nat. Prod. Res. 2008, 10, 616.

[14] Cong, Y. Ph.D. Dissertation, Shenyang Pharmaceutical University, Shenyang, 2007. (丛悦, 博士论文, 沈阳药科大学, 沈阳, 2007.)

[15] Zhang, X.; Song, F.-R.; Wang, L.-S.; Liu, Z.-Q.; Liu, S.-Y. Acta Chim. Sinica 2007, 65, 829. (张旭, 宋凤瑞, 王隶书, 刘志强, 刘淑莹, 化学学报, 2007, 65, 829.)

[16] Tang, J.; Li, H.-L.; Huang, H.-Q.; Zhang, W.-D. Prog. Pharm. Sci. 2006, 30, 206. (汤建, 李慧梁, 黄海强, 张卫东, 药学进展, 2006, 30, 206.)

[17] Wang, B. M.S. Thesis, Second Military Medical University, Shanghai, 2007. (王斌, 硕士论文, 第二军医大学, 上海, 2007.)

[18] Li, D.-X.; Deng, W.-L. Pharmacol. Clin. Chin. Med. 2009, 25, 93. (李东晓, 邓文龙, 中药药理与临床, 2009, 25, 93.)

[19] Meng, X.-S.; Kang, T.-G.; Ye, T.-X.; Wang, J.; Luo, G.-A. Chin. Arch. Tradit. Chin. Med. 2011, 28, 754. (孟宪生, 康廷国, 叶挺祥, 王静, 罗国安, 中华中医药学刊, 2011, 28, 754.)

[20] Wang, Y.-G.; Wang, C.; Liang, Q.-D.; Rang, W.-Q.; Xiao, C.-R.; Gao, Y. Sci. China, Life Sci. 2011, 41, 925. (王宇光, 王超, 梁乾德, 让蔚清, 肖成荣, 高月, 中国科学: 生命科学, 2011, 41, 925.)

UPLC-Q-TOF/MS分析不同比例丹参配伍藜芦毒性生物碱变化规律

Regularity of Toxic Alkaloids during the Combination of Veratrum nigrum and Salvia miltiorrhiza by UPLC-Q-TOF/MS

PDF

Supporting Info.

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments