F-NYWQ (perfluoroootyl-amidopropyl-monoethylogy bonded silica) has been used as a column packing for HPLC separation of oholanio acids and esters in the normal phase mode.A direct determination of the oholanio acids was varried out by using a flame ionization detector,or alternatively the acids were transformed to their p-bromophenaoyl esters and followed by determination with UV deteotor.In the latter method seven oholanio esters in which five are dihydrogy derivatives namely deogy oholio acid (5),ohenodeogyoholio avid (6),ursodeogy oholio acid (7),3β,6β-dihydrogy-5α-vholanio acid (8) and hyodeogyoholio acid (9) can be fully separated.It takes only ll minutes for the separation.