3-phthalimido-2-ono-butyraldehyde bisthiosemicarbazone (6) (namely Tai-Ding-An,V 6133) exhibited marked inhibiting activity against Chlamydozoa trachamatis and some species of bacteria in vitro as well as against Walker 256 carcinoma in animals.It showed definite therapeutic e}caoy in the treatment of various Chlamydoazoa trachomatis and some virus infected skin diseases in clinical trials.