Studies on the Interaction between Rare-earth Salts of Heteropoly EuHSiMo10W2O40· 25H2O and Bovine Serum Albumin

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (16): 1519-1523. Previous Articles Next Articles

稀土杂多酸盐EuHSiMo₁₀W₂O₄₀·25H₂O与BSA相互作用的研究

胡艳军, 刘义, 侯安新, 赵儒铭, 屈啸声, 屈松生

武汉大学化学与分子科学学院, 武汉, 430072

投稿日期:2003-11-13 修回日期:2004-04-30 发布日期:2014-02-17
通讯作者: prof.liuyi@263.net
作者简介:刘义:E-mail:prof.liuyi@263.net;Tel:027-87218304;Fax:027-87647617
基金资助:
国家自然科学基金(No.20373051)和教育部高校优秀青年教师教学科研奖励计划(2001)资助项目.

Studies on the Interaction between Rare-earth Salts of Heteropoly EuHSiMo₁₀W₂O₄₀· 25H₂O and Bovine Serum Albumin

HU Yan-Jun, LIU Yi, HOU An-Xin, ZHAO Ru-Ming, QU Xiao-Sheng, QU Song-Sheng

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072

Received:2003-11-13 Revised:2004-04-30 Published:2014-02-17

Abstract

The rare-earth salts of heteropoly acid, EuHSiMo₁₀W₂O₄₀·25H₂O (EuW₂), have been synthesized and characterized.The binding reaction between EuW₂ and bovine serum albumin (BSA) was investigated by fluorescence and UV-vis absorption spectroscopy.It is proved that the fluorescence quenching of BSA by EuW₂ is a result of the formation of BSA-EuW₂ complex.The binding site number n, apparent binding constant K and corresponding thermodynamic parameters were measured at different temperatures by fluorescence quenching method.The binding distance r between EuW₂ and BSA was obtained according to Förster theory of non-radiation energy transfer.The effect of EuW₂ on the conformation of BSA was analyzed using synchronous fluorescence spectroscopy.

Key words: rare2earth salt of heteropoly acid, bovine serum albumin, fluorescence quenching, spectroscopic methodology, thermodynamic parameter

Cite this article

HU Yan-Jun, LIU Yi, HOU An-Xin, ZHAO Ru-Ming, QU Xiao-Sheng, QU Song-Sheng. Studies on the Interaction between Rare-earth Salts of Heteropoly EuHSiMo₁₀W₂O₄₀· 25H₂O and Bovine Serum Albumin[J]. Acta Chimica Sinica, 2004, 62(16): 1519-1523.

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稀土杂多酸盐EuHSiMo₁₀W₂O₄₀·25H₂O与BSA相互作用的研究

Studies on the Interaction between Rare-earth Salts of Heteropoly EuHSiMo₁₀W₂O₄₀· 25H₂O and Bovine Serum Albumin

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稀土杂多酸盐EuHSiMo10W2O40·25H2O与BSA相互作用的研究