Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (19): 1922-1926. Previous Articles     Next Articles

原位聚合那格列奈分子印迹手性固定相的分子识别特性研究

尹俊发1,2, 杨更亮1,2, 张轶华2, 刘海燕2, 陈义1   

  1. 1. 中国科学院化学研究所分子科学中心, 北京, 100080;
    2. 河北大学药学院, 保定, 071002
  • 投稿日期:2004-02-10 修回日期:2004-06-01 发布日期:2014-02-17
  • 通讯作者: 杨更亮,E-mail:ygl@mail.hbu.edu.cn;Tel:(0312)5079788 E-mail:ygl@mail.hbu.edu.cn
  • 基金资助:
    国家自然科学基金(No.20375010)、河北省自然科学基金(No.202096)、科学技术部基金(Nos.2002CCA3100,KJCX2-H4)和中国科学院百人计划资助项目.

Molecular Recognition in in-situ Nateglinide Imprinted Chiral Stationary Phase

YIN Jun-Fa1,2, YANG Geng-Liang1,2, ZHANG Yi-Hua2, LIU Hai-Yan2, CHEN Yi1   

  1. 1. Molecular Science Center, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080;
    2. College of Pharmacy, Hebei University, Baoding 071002
  • Received:2004-02-10 Revised:2004-06-01 Published:2014-02-17

Nateglinide has been employed as template for the in-situ preparation of molecularly imprinted continuous rod applied as liquid chromatographic chiral stationary phases for the separation of the enantiomers of the nateglinide. It was shown that the nateglinide-imprinted polymer (nateglinide-MIP) was capable of recognizing the enantiomeric difference of the template and its L-enantiomer, while the non-imprinted polymer had no such ability. The thermodynamic parameters in the chiral separation process were cauculated, and Scatchard analysis was performed to investigate the thermodynamic characteristics and binding characteristics of the nateglinide-MIP stationary phase. The excellent enantioselectivities for the imprinted substance, together with the continuous and homogenous structures of the in-situ MIP, boded well their use as chiral stationary phase.

Key words: molecularly imprinted polymer, chiral stationary phase, in-situ polymerization, chiral separation, nateglinide