Chemical Constituents with Neuraminidase Inhibitory Actives from Campylotropis Hirtella

doi:10.6023/A15030209

Acta Chim. Sinica ›› 2015, Vol. 73 ›› Issue (7): 741-748.DOI: 10.6023/A15030209 Previous Articles Next Articles

Article

毛秔子梢中天然高活性的神经氨酸酶抑制成分的研究

杜昕^a, 宣碧霞^a, 沈征武^a,b

a 上海中医药大学中药学院上海 201203;
b 上海交通大学医学院上海 200025

投稿日期:2015-03-26 发布日期:2015-04-03
通讯作者: 沈征武 E-mail:jeff_shen_1999@yahoo.com
基金资助:
项目受上海市生物医药领域科技支撑项目(No. 14401900600)资助.

Chemical Constituents with Neuraminidase Inhibitory Actives from Campylotropis Hirtella

Du Xin^a, Xuan Bixia^a, Shen Zhengwu^a,b

a School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203;
b Shanghai Jiaotong University, School of Medicine, Shanghai 200025

Received:2015-03-26 Published:2015-04-03
Supported by:
Supporting information for this article is available free of charge via the Internet at http://sioc-journal.cn.Project supported by the Science & Technology commission of Shanghai municipal government (No.14401900600).

Influenza is and remains a disease to reckon with. Seasonal epidemics around the world kill several hundred thousand people every year. Development of effective drugs for the treatment or prevention of epidemic and pandemic influenza is important in order to reduce its impact. Neuraminidase inhibitors is a class of anti-influenza drugs available for influenza therapy currently. However, emergence of resistance to these drugs has been detected, which raises concerns regarding their widespread use. Therefore, the search for low toxic natural neuraminidase inhibitor is one of the route for finding new anti-influenza virus drugs. In current study, the classical MUNANA-based NA activity assay was established to assess the neuraminidase inhibitory activities of compounds isolated from Campylotropis hirtella. The bioassay guided separation of 95% ethanol extract of the dried root of C. hirtella has led to the isolation of ten compounds, and their structures were elucidated on the basis of spectroscopic methods, with special emphasis on 1D and 2D NMR techniques. Compounds 1～4 are new compounds and these are two chromones, an isoflavone and a glycosides. Compounds 5～10 were isolated from C. hirtella for the first time. All compounds were assesses for their inhibitory activities against influenza A1(H1N1) virus NA. Compounds 1, 2, 3, 5 showed good and dose dependent inhibitory effects against NA enzyme. Among them, compound 1 had the best NA inhibitory activity with the IC₅₀ at 16.76 μmol/L. Although, compound 1 is less active than Tamiflu, however, the results from the study demonstrated that isoflavone and chromone could be potent neuraminidase inhibitor. Since the structure of compound 1 is very similar to Hirtellanine B, an isoflavonoid isolated from the same herb with potent antiproliferative effects in cancer cells, the biosynthetic pathway of both compound 1 and Hirtellanine B were proposed.

Key words: Radix Campylotropis hirtella, chemical constituent, NA inhibitors, influenza virus

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Du Xin, Xuan Bixia, Shen Zhengwu. Chemical Constituents with Neuraminidase Inhibitory Actives from Campylotropis Hirtella[J]. Acta Chim. Sinica, 2015, 73(7): 741-748.

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毛秔子梢中天然高活性的神经氨酸酶抑制成分的研究

Chemical Constituents with Neuraminidase Inhibitory Actives from Campylotropis Hirtella

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