Preparation of Free-standing Micropatterned Keratin Films by Soft Lithography

doi:10.6023/A19020060

Acta Chim. Sinica ›› 2019, Vol. 77 ›› Issue (6): 533-538.DOI: 10.6023/A19020060 Previous Articles Next Articles

Article

软光刻法制备具有表面微结构的角蛋白膜

朱水洪^a, 罗文昊^a, 曾文斌^a, 林友辉^a, 刘向阳^a,b

a 厦门大学物理系生物仿生及软物质研究院福建省柔性功能材料重点实验室厦门 361005;
b 新加坡国立大学物理系新加坡 117542

投稿日期:2019-02-11 发布日期:2019-04-29
通讯作者: 林友辉, 刘向阳 E-mail:linyouhui@xmu.edu.cn;phyliuxy@nus.edu.sg
基金资助:
项目受国家自然科学基金（Nos.21771150，21401154，U1405226）、111计划（No.B16029）、广东省自然科学基金（No.2014A030310005）及中央高校基本业务费（No.20720170011）资助.

Preparation of Free-standing Micropatterned Keratin Films by Soft Lithography

Zhu Shuihong^a, Luo Wenhao^a, Zeng Wenbin^a, Lin Youhui^a, Liu Xiang Yang^a,b

a Department of Physics, Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Xiamen University, Xiamen 361005, China;
b Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore, 117542

Received:2019-02-11 Published:2019-04-29
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21771150, 21401154, U1405226), the 111 Project (No. B16029), the Natural Science Foundation of Guangdong Province (2014A030310005) and the Fundamental Research Funds for the Central Universities of China (No. 20720170011).

1. Preparation of Free-standing Micropatterned Keratin Films by Soft Lithography.PDF(269KB)

Abstract

Recently, the use of micro-nano manufacturing processes to fabricate high-precision spatial patterns of proteins or peptides has provided important applications in cell biology, tissue engineering, pharmaceutical science, and optoelectronics. As a natural biological protein, wool keratin (WK) have excellent water solubility, good biocompatibility, and controllable degradability. However, WK usually cannot self-assemble to form a gel network or other insoluble forms. Therefore, it is difficult to prepare molded WK materials, such as a fiber, a film, and a gel. To solve this problem, this paper explores the feasibility of preparing photocrosslinkable WK. WK was extracted from wool fibres, and its side groups were reacted with the reagent 2-isocyanatoethyl methacrylate (IEM), yielding a photoactive WK precursor. And then, WK films with patterned microstructures were obtained by a covalent cross-linking method. This method can also be used to obtain other forms of WK materials. The as-prepared WK films were characterized by 3D laser scanning microscopy, UV-visible near-infrared spectroscopy and Fourier transform infrared microscopy. The experimental results showed that after two pattern shifts, the pattern on the WK film still maintained good integrity and conformed to the original pattern on the silicon wafer, which indicated that the pattern transfer method can achieve perfect reproduction of the pattern. In addition, we also demonstrated that the formation of structural colors caused by periodically arranged microstructures on WK films. Our experimental results not only provide a facile method to prepare WK films with surface microstructures by soft lithography but also give a new way for the preparation of molded WK. We expect the good optical properties and controlled degradation properties of WK open up new directions for the manufacture of biodegradable optics and implantable flexible microelectronic devices.

Key words: wool keratin, soft lithography, covalent cross-linking, patterns, surface microstructure

Cite this article

Zhu Shuihong, Luo Wenhao, Zeng Wenbin, Lin Youhui, Liu Xiang Yang. Preparation of Free-standing Micropatterned Keratin Films by Soft Lithography[J]. Acta Chim. Sinica, 2019, 77(6): 533-538.

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软光刻法制备具有表面微结构的角蛋白膜

Preparation of Free-standing Micropatterned Keratin Films by Soft Lithography

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