Preparation and Preliminary Study on Immune Function of Mannose-modified Micromotor★

doi:10.6023/A23040151

Acta Chimica Sinica ›› 2023, Vol. 81 ›› Issue (7): 741-748.DOI: 10.6023/A23040151 Previous Articles Next Articles

Special Issue: 庆祝《化学学报》创刊90周年合辑

Article

甘露糖修饰的微马达的制备及其免疫功能初探^★

杜思南^a, 赵丽曼^a^,^*(), 张泽新^b, 陈国颂^a^,^*()

a 复旦大学高分子科学系聚合物分子工程国家重点实验室上海 200433
b 苏州大学材料与化学化工学部软凝聚态物理及交叉研究中心苏州 215123

投稿日期:2023-04-20 发布日期:2023-06-16
作者简介:
^★庆祝《化学学报》创刊90周年.
基金资助:
国家重点研发计划项目(2022YFB3804401); 国家自然科学基金(52125303); 国家自然科学基金(51721002); 国家自然科学基金(91956127); 上海市教委科研创新计划项目(2023ZKZD02)

Preparation and Preliminary Study on Immune Function of Mannose-modified Micromotor^★

Sinan Du^a, Liman Zhao^a(), Zexin Zhang^b, Guosong Chen^a()

a The State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science of Fudan University, Shanghai 200433
b College of Chemistry, Chemical Engineering and Materials Science and Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215123

Received:2023-04-20 Published:2023-06-16
Contact: *E-mail: lmzhao@fudan.edu.cn; guosong@fudan.edu.cn
About author:
^★Dedicated to the 90th anniversary of Acta Chimica Sinica.
Supported by:
National Key Research and Development Program of China(2022YFB3804401); National Natural Science Foundation of China(52125303); National Natural Science Foundation of China(51721002); National Natural Science Foundation of China(91956127); Innovation Program of Shanghai Municipal Education Commission(2023ZKZD02)

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Abstract

In order to develop a novel sugar hybrid material with self-actuated micromotor and immune function, we designed and prepared a dumbbell micromotor modified with mannose (Zinc oxide/Polydopamine/Mannose micromotor, ZnO/PDA/Man micromotor for short), which combines the kinetic properties of ZnO active colloid and visible light absorption properties of PDA materials. X-ray diffraction (XRD) and Raman spectroscopy results showed that the introduction of PDA and Man does not change the crystal shape of ZnO, which ensures that the photocatalytic activity of ZnO is unaffected in ZnO/PDA/Man. The dumbbell structure of the micromotor can cause an uneven distribution of ions, the ion gradient will drive micromotor to move. The micromotor energy source is non-toxic and can move itself in solution with pure water as fuel under visible light. The simulation verified that the motion mechanism of dumbbell micromotor is ionic self-diffusiophoresis, which keeps the head in front and pulls the colloid to move. The motion behavior of the micromotor under different intensities of visible light was observed and recorded by the microscope. It was found that the micromotor changed from translational motion to circular motion with the enhancement of light intensity, and the micromotor's motion speed also increased gradually. Meanwhile, by adjusting the direction of incident light, the motion direction of ZnO/PDA/Man micromotor can be accurately adjusted to make it move according to the predetermined route. In order to preliminarily explore whether the mannose-modified micromotor can regulate the phenotype of macrophages, the ZnO/PDA/Man micromotor was incubated with macrophages, and the morphological changes of macrophages were observed with a microscope. The results showed that the micromotor could be used as an immune activator to polarize macrophages. What'more, cytotoxicity experiment indicated that ZnO/PDA/Man micromotor has good biocompatibility. Compared with traditional immune activators, ZnO/PDA/Man micromotor is expected to realize free movement in tumor tissues under visible light irradiation, so as to promote their deep penetration into tumor tissues, which has potential application in the field of tumor immunotherapy.

Key words: mannose, micromotor, visible light, macrophage, tumor immunity

Cite this article

Sinan Du, Liman Zhao, Zexin Zhang, Guosong Chen. Preparation and Preliminary Study on Immune Function of Mannose-modified Micromotor^★[J]. Acta Chimica Sinica, 2023, 81(7): 741-748.

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Fig. & Tab. 6

Figure 1. Preparation route of ZnO/PDA/Man micromotor

Figure 2. (a~c) SEM and (d~f) TEM images of ZnO based micromotors

Figure 3. (a) TGA curves, (b) XRD patterns and (c) Raman spectra of ZnO based micromotors

Figure 4. Superimposed time-delay snapshot and tracks of ZnO/PDA/Man micromotors moving under visible light irradiation of (a) 2.4 mW/cm2 and (b) 12.9 mW/cm2; (c) motion mechanism, (d) surrounding electric field distribution and potential simulation diagram, (e) surrounding velocity field distribution simulation diagram of ZnO/PDA/Man micromotors

Figure 5. Trajectories of ZnO/PDA/Man micromotors moving in pure water for 10 s under visible light irradiation of (a) 2.4 mW/cm2, (b) 5.9 mW/cm2, and (c) 12.9 mW/cm2; (d) the trajectory of movement in pure water for 5 min under visible light irradiation of 25.2 mW/cm2; (e) average velocity curve of micromotor with light intensity; (f) trajectory diagram of micromotor movement direction precisely controlled by light field (Purple arrow indicates the direction of incident light). Scale bar: 10 μm

Figure 6. (a) Cytotoxicity assessment of ZnO-based micromotors and (b) microscopic images of micromotors after incubation with macrophages for 24 h. Scale bar: 10 μm

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甘露糖修饰的微马达的制备及其免疫功能初探^★

Preparation and Preliminary Study on Immune Function of Mannose-modified Micromotor^★

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甘露糖修饰的微马达的制备及其免疫功能初探★

Preparation and Preliminary Study on Immune Function of Mannose-modified Micromotor★

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PDF

Supporting Info.

Knowledge

Abstract

Cite this article

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Fig. & Tab. 6

References 45

Related Articles 15

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甘露糖修饰的微马达的制备及其免疫功能初探^★

Preparation and Preliminary Study on Immune Function of Mannose-modified Micromotor^★